Detailed information of TA system TA027096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 952122..953359
• Replicon: chromosome
• Accession: NC_017592
• Organism: Streptococcus pneumoniae OXC141

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A4J1ZCF4
• Locus tag: SPNOXC_RS05115
• Protein ID: WP_000405368.1
• Coordinates: 952598..953359 (+)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: SPNOXC_RS05110
• Protein ID: WP_000579602.1
• Coordinates: 952122..952598 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SPNOXC_RS05085	947195..948049	+	855	WP_000900019.1	ADP-ribosylglycohydrolase family protein	-
SPNOXC_RS05090	948161..949045	+	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
SPNOXC_RS05095	949208..950947	+	1740	WP_000423464.1	glycoside hydrolase family 13 protein	-
SPNOXC_RS05105	951222..951677	+	456	WP_000500478.1	hypothetical protein	-
SPNOXC_RS05110	952122..952598	+	477	WP_000579602.1	helix-turn-helix domain-containing protein	Antitoxin
SPNOXC_RS05115	952598..953359	+	762	WP_000405368.1	zeta toxin family protein	Toxin
SPNOXC_RS05120	953387..954544	+	1158	WP_001213951.1	hypothetical protein	-
SPNOXC_RS05125	955376..955735	+	360	WP_000529545.1	hypothetical protein	-
SPNOXC_RS05130	955745..956110	+	366	WP_000263039.1	MobC family plasmid mobilization relaxosome protein	-
SPNOXC_RS12880	956097..957947	+	1851	Protein_982	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	936866..982339	45473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29185.27 Da
• Isoelectric Point: 7.5090

>T27096 WP_000405368.1 NC_017592:952598-953359 [Streptococcus pneumoniae OXC141]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFVGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
RVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T27096 NC_017592:952598-953359 [Streptococcus pneumoniae OXC141]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATATACCAAAGATTTTGTAGGAAAAATGGTAGAGTCTTTAGTAACAAAATTGAGTAG
TTTGAGATACAATCTTTTGATCGAGGGAACTTTACGAACAGTTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAATA
AGGGATATGAAGTACAATTGGCCTTAATTGCGACAAAGCCTGAATTGTCGTATCTAAGTACTCTTATCCGTTATGAAGAA
CTGTACATTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATTTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTCTTTGGGGAGTGGAGTCAGGTAGAGAAGGAGATGTTG
AGGGTAGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18289.70 Da
• Isoelectric Point: 4.4188

>AT27096 WP_000579602.1 NC_017592:952122-952598 [Streptococcus pneumoniae OXC141]
MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT27096 NC_017592:952122-952598 [Streptococcus pneumoniae OXC141]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACACGAATTTGCACGGATTATAGGTATTTC
ACGAAATAGTCTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCAGACAAATATCTATTTAGTAGAAAATTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A4J1ZCF4