Detailed information of TA system TA027092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 206731..207452
• Replicon: plasmid pTTJL1801
• Accession: NC_017588
• Organism: Thermus thermophilus JL-18

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: TTJL18_RS11315
• Protein ID: WP_014630570.1
• Coordinates: 206731..207039 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: TTJL18_RS11320
• Protein ID: WP_014511247.1
• Coordinates: 207036..207452 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TTJL18_RS11290	202974..203153	+	180	WP_014511253.1	hypothetical protein	-
TTJL18_RS11295	203163..204332	+	1170	WP_014630566.1	cytochrome P450	-
TTJL18_RS11300	204329..205027	+	699	WP_014630567.1	lycopene cyclase domain-containing protein	-
TTJL18_RS11305	205032..205706	+	675	WP_014630568.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
TTJL18_RS11310	205703..206725	+	1023	WP_014630569.1	glycosyltransferase	-
TTJL18_RS11315	206731..207039	+	309	WP_014630570.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
TTJL18_RS11320	207036..207452	+	417	WP_014511247.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
TTJL18_RS11325	207550..209118	+	1569	WP_014630571.1	phytoene desaturase	-
TTJL18_RS11330	209115..210113	+	999	WP_014630572.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
TTJL18_RS11335	210123..210515	+	393	WP_014511244.1	nucleotidyltransferase domain-containing protein	-
TTJL18_RS11340	210502..210915	+	414	WP_014630573.1	DUF86 domain-containing protein	-
TTJL18_RS11345	210912..211859	-	948	WP_014630574.1	DUF523 and DUF1722 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..265886	265886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (20-83)

Antitoxin

• PIN domain (3-129)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11640.40 Da
• Isoelectric Point: 8.5043

>T27092 WP_014630570.1 NC_017588:206731-207039 [Thermus thermophilus JL-18]
VASKLDRPVFSVYPEGVKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKT
LPNEEVEALFQRQKTGFREVDF

Nucleotide

• Length: 309 bp

>T27092 NC_017588:206731-207039 [Thermus thermophilus JL-18]
GTGGCCTCTAAACTAGACAGACCGGTCTTTTCTGTGTACCCTGAGGGGGTGAAGGCCATCTGGCAACTCCAAGAGGCCAA
AGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCAGCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGG
TGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCTCGGTCCTCGAGGCCCTAAGACCCCCCAAGACC
CTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGCTTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15241.82 Da
• Isoelectric Point: 10.4393

>AT27092 WP_014511247.1 NC_017588:207036-207452 [Thermus thermophilus JL-18]
MRYLLDTNVVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAE
VMELWGELTGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 417 bp

>AT27092 NC_017588:207036-207452 [Thermus thermophilus JL-18]
CTGAGGTACCTGCTGGACACCAACGTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCG
CCACCTTAAAGTGGGGGAGGCGTACCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGG
CCCGAAGGGCCATCCTCAGCCGATGGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAG
GTGATGGAGCTCTGGGGCGAGCTCACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGC
GGCCACGGCCCTGCGGCACGGCCTGATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGA
ACCCCTGGGAAGGGTAA