Detailed information of TA system TA027086

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3547187..3547765
• Replicon: chromosome
• Accession: NC_017584
• Organism: Rhodospirillum rubrum F11

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q2RPR9
• Locus tag: F11_RS15795
• Protein ID: WP_011390829.1
• Coordinates: 3547187..3547483 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q2RPR8
• Locus tag: F11_RS15800
• Protein ID: WP_011390830.1
• Coordinates: 3547484..3547765 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F11_RS15775	3543095..3543844	+	750	WP_011390825.1	electron transfer flavoprotein subunit beta/FixA family protein	-
F11_RS15780	3543846..3544781	+	936	WP_011390826.1	FAD-binding protein	-
F11_RS15785	3544824..3545696	+	873	WP_011390827.1	3-hydroxybutyryl-CoA dehydrogenase	-
F11_RS15790	3545804..3547000	+	1197	WP_011390828.1	glycosyltransferase family 39 protein	-
F11_RS15795	3547187..3547483	+	297	WP_011390829.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F11_RS15800	3547484..3547765	+	282	WP_011390830.1	putative addiction module antidote protein	Antitoxin
F11_RS15805	3547837..3548391	-	555	WP_011390831.1	NAD(P)H-dependent oxidoreductase	-
F11_RS15810	3548482..3549984	-	1503	WP_011390832.1	ribosome biogenesis GTPase Der	-
F11_RS15815	3550079..3551446	-	1368	WP_011390833.1	PQQ-binding-like beta-propeller repeat protein	-
F11_RS15820	3551436..3552197	-	762	WP_011390834.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-89)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10751.35 Da
• Isoelectric Point: 7.9765

>T27086 WP_011390829.1 NC_017584:3547187-3547483 [Rhodospirillum rubrum F11]
MFDVRQTEAFEEWLAGLADEKAGARIAQRIVRLRNGLFGDVKPVGDGVSELRVDDGPGYRLYFVQRGTTLIILLCGGIKG
AQRRDIARAKAMAAEVES

Nucleotide

• Length: 297 bp

>T27086 NC_017584:3547187-3547483 [Rhodospirillum rubrum F11]
TTGTTTGATGTCCGCCAGACCGAGGCGTTTGAAGAGTGGCTCGCGGGCTTGGCCGATGAAAAGGCCGGCGCGCGCATCGC
TCAACGCATCGTGCGACTGCGCAACGGCCTGTTCGGCGATGTGAAGCCGGTGGGCGATGGGGTATCGGAGCTGCGAGTCG
ACGATGGCCCAGGCTACCGGTTGTACTTCGTACAACGGGGAACGACCCTCATCATCTTGCTGTGTGGCGGCATCAAAGGG
GCCCAGCGCCGTGATATCGCCAGAGCGAAGGCGATGGCCGCAGAAGTGGAAAGCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 9832.14 Da
• Isoelectric Point: 5.7973

>AT27086 WP_011390830.1 NC_017584:3547484-3547765 [Rhodospirillum rubrum F11]
MIKTKPFDAARHLTSPEAQQDLLNDAFASGSASYIADALGVIARARGMTDVAREAGVTREALYKSLSEDGDPRLTTLLGV
ARALGVTLTARLD

Nucleotide

• Length: 282 bp

>AT27086 NC_017584:3547484-3547765 [Rhodospirillum rubrum F11]
ATGATCAAAACCAAGCCCTTCGATGCCGCACGTCATCTGACCTCGCCGGAAGCCCAGCAAGACCTGCTTAACGATGCCTT
CGCCAGCGGCAGCGCGTCCTATATCGCCGACGCCCTTGGCGTGATCGCCCGGGCTCGCGGAATGACGGATGTGGCACGCG
AGGCGGGCGTGACGCGAGAGGCGCTGTATAAATCGCTAAGCGAAGACGGCGATCCGCGCCTGACAACCCTTCTGGGCGTC
GCCCGGGCATTGGGGGTGACTCTGACCGCCCGTCTTGATTAA

Structures

Toxin

• AlphaFold DB: Q2RPR9

Antitoxin

• AlphaFold DB: Q2RPR8