Detailed information of TA system TA027075

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sezT-pezT/zeta-couple_hipB
• Location: 558107..559353
• Replicon: chromosome
• Accession: NC_017581
• Organism: Streptococcus thermophilus JIM 8232

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: F8LVX2
• Locus tag: STH8232_RS03015
• Protein ID: WP_014621295.1
• Coordinates: 558583..559353 (+)
• Length: 257 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: STH8232_RS03010
• Protein ID: WP_014621294.1
• Coordinates: 558107..558583 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
STH8232_RS02975	554577..554876	+	300	WP_014621288.1	hypothetical protein	-
STH8232_RS02980	554890..555180	+	291	WP_014621289.1	hypothetical protein	-
STH8232_RS02985	555276..555914	+	639	WP_014621290.1	hypothetical protein	-
STH8232_RS02990	555954..557030	+	1077	WP_014621291.1	toprim domain-containing protein	-
STH8232_RS02995	557084..557311	+	228	WP_012775533.1	hypothetical protein	-
STH8232_RS03000	557308..557697	+	390	WP_014621292.1	hypothetical protein	-
STH8232_RS03005	557747..558037	+	291	WP_014621293.1	hypothetical protein	-
STH8232_RS03010	558107..558583	+	477	WP_014621294.1	helix-turn-helix domain-containing protein	Antitoxin
STH8232_RS03015	558583..559353	+	771	WP_014621295.1	zeta toxin family protein	Toxin
STH8232_RS03020	559367..561613	+	2247	WP_014621296.1	AAA family ATPase	-
STH8232_RS03025	561617..563632	+	2016	WP_014621297.1	ATP-dependent helicase	-
STH8232_RS03030	563977..564336	+	360	WP_014621298.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 257 a.a.
• Molecular weight: 29362.24 Da
• Isoelectric Point: 5.2881

>T27075 WP_014621295.1 NC_017581:558583-559353 [Streptococcus thermophilus JIM 8232]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDEPKAILLGRQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLASKGYQVSLAVIGTKPELSYLSTLIRYEE
LYVIDPTQARATPKEHHDGIVENLVDNLRELESEKVFEQIQIYQRDRACIYDSETDEDSAAEVLQECLFGKWSKVEEEML
KVGRERLREFSVMKEN

Nucleotide

• Length: 771 bp

>T27075 NC_017581:558583-559353 [Streptococcus thermophilus JIM 8232]
ATGAGGTTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAACGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATGAACCGAAAGCTATCTTGCTTGGTAGACAAAGCGGAGCAGGTAAGACAACGATACATCGTATCAAGCAAA
AGGAATTTCAAGGCAATATCATTATCATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCACTGCAAGAA
AAGTATGGCAAGGACAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGCAC
ACAAGGCTACCATTTGCTGATTGAAGGGACTTTGCGTACCACTCAAGTTCCTCGTCAGACTGCTCAATTATTAGCTTCGA
AAGGATACCAAGTTTCTTTAGCAGTGATTGGTACCAAACCAGAACTGTCCTATCTCAGTACCTTGATTCGTTACGAAGAG
CTTTATGTCATAGATCCGACTCAGGCCAGAGCAACCCCAAAAGAACACCATGATGGGATTGTAGAGAACTTGGTGGATAA
CCTAAGGGAGTTAGAAAGTGAGAAAGTCTTTGAACAAATTCAAATTTACCAAAGAGATAGGGCTTGTATCTATGATTCTG
AAACTGATGAAGACTCCGCGGCAGAAGTGCTACAAGAATGCCTCTTCGGAAAATGGAGTAAGGTAGAGGAAGAGATGTTG
AAGGTGGGACGAGAACGGTTGAGAGAGTTTTCAGTAATGAAAGAAAATTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18287.70 Da
• Isoelectric Point: 4.3971

>AT27075 WP_014621294.1 NC_017581:558107-558583 [Streptococcus thermophilus JIM 8232]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYQLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDETNPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT27075 NC_017581:558107-558583 [Streptococcus thermophilus JIM 8232]
ATGATTGGAGACAATATTAAATCACTACGCCGAACACACGATTTAACACAACCAGAATTTGCGAAAATGGTCGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGAACCAGCACCGTTTCAACAGAACTCATCGATCGTATTTGTCAGAAATTTA
ACGTCTCTTATGTTGATATTGTAGGGGAGGATAAGATGCTAACACCTGTTGAAGATTACCAACTAACCTTAAAAGTAGAA
GTGATAAAAGAACGTGGCGCAGCCATTTTATCACAGCTTTATAGATACCAGGATAGTCAGGATATTGCTTTTGATGATGA
AACCAATCCTTGGATTCTCATGAGTGATGACTTAGCTGAATTGATTAATACAAAGATTTACCTTGTCGATACCTTCGATG
AAATCGAGCGTTACAATGGCTATTTAGATGGTATTGAGCGGATGTTAGATATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: F8LVX2