Detailed information of TA system TA027068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 15190..16208
• Replicon: plasmid pSBAL11703
• Accession: NC_017578
• Organism: Shewanella baltica OS117

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: SBAL117_RS24330
• Protein ID: WP_011840000.1
• Coordinates: 15639..16208 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: SBAL117_RS24325
• Protein ID: WP_011839999.1
• Coordinates: 15190..15639 (+)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL117_RS24275	10405..10881	+	477	WP_011839993.1	DUF417 family protein	-
SBAL117_RS24280	10979..11560	-	582	WP_011839994.1	DUF2913 family protein	-
SBAL117_RS24285	11557..11835	-	279	WP_011839995.1	hypothetical protein	-
SBAL117_RS25265	12394..12693	+	300	WP_014620443.1	hypothetical protein	-
SBAL117_RS24305	12653..13582	+	930	WP_014620444.1	integrase	-
SBAL117_RS24310	14134..14409	-	276	WP_148258346.1	hypothetical protein	-
SBAL117_RS24315	14424..14678	-	255	WP_011839997.1	Txe/YoeB family addiction module toxin	-
SBAL117_RS24320	14675..14926	-	252	WP_011839998.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
SBAL117_RS24325	15190..15639	+	450	WP_011839999.1	helix-turn-helix domain-containing protein	Antitoxin
SBAL117_RS24330	15639..16208	+	570	WP_011840000.1	PIN domain-containing protein	Toxin
SBAL117_RS24335	16271..17476	-	1206	WP_014620445.1	IS256-like element ISSod5 family transposase	-
SBAL117_RS24340	17697..20696	-	3000	WP_014620446.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71173	71173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• Helix-turn-helix domain, group 17 (79-126)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21664.22 Da
• Isoelectric Point: 5.2836

>T27068 WP_011840000.1 NC_017578:15639-16208 [Shewanella baltica OS117]
MASYTVVFDACVMYPAPLRSYLMYLANAGLFRARWTEQIHDEWMRNVLLNNPRITEAMLERTKQLINENVPDCLIEGYEP
LIKGLTLPDMNDRHVVAAALKGHAEAIITFNLKDFPASELNILELSAIHPDEFLCDMFELDLSSCIKAAQQQRSSLKNPP
MTTSEYLNCLQKQRLPVFVSKLRPFELML

Nucleotide

• Length: 570 bp

>T27068 NC_017578:15639-16208 [Shewanella baltica OS117]
ATGGCTTCGTATACTGTTGTTTTTGATGCGTGTGTCATGTATCCCGCACCACTTCGTAGTTATTTGATGTATTTAGCCAA
TGCGGGCTTATTTAGGGCTCGTTGGACTGAGCAGATCCATGATGAATGGATGCGTAATGTATTGTTGAATAATCCTCGCA
TCACAGAAGCAATGTTAGAACGTACGAAACAACTGATTAATGAAAATGTACCTGATTGCCTTATCGAAGGATATGAGCCG
TTAATTAAAGGATTAACACTGCCTGATATGAATGATAGGCATGTGGTTGCTGCGGCTTTAAAAGGGCATGCAGAGGCCAT
CATAACGTTTAATCTTAAAGATTTTCCTGCATCAGAGCTCAATATACTTGAACTATCAGCTATCCATCCAGATGAGTTTT
TGTGCGACATGTTTGAGTTAGATCTAAGCTCTTGTATCAAAGCTGCTCAACAGCAAAGAAGTAGCTTAAAGAATCCCCCG
ATGACAACATCAGAGTATTTAAATTGCTTACAAAAGCAAAGGTTACCGGTTTTTGTTTCGAAGCTGAGGCCATTTGAATT
AATGCTATAG

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16606.03 Da
• Isoelectric Point: 5.2075

>AT27068 WP_011839999.1 NC_017578:15190-15639 [Shewanella baltica OS117]
MNMTTLPNAEEIAMARLGSQELSAVIESNGEAQQISVIDKQGQSHDVTLPSSVLKMMIEVLTQLGQGNSVNITPIHAEMT
TQQGADFLNMSRPTFIKLLDSREIPFTRVGNRRKVPFSAVIKYKQQLEIQRLSTLDELSRLDQELGMGY

Nucleotide

• Length: 450 bp

>AT27068 NC_017578:15190-15639 [Shewanella baltica OS117]
ATGAACATGACTACTTTGCCGAACGCCGAAGAAATCGCAATGGCTCGGTTGGGCAGCCAGGAGTTATCTGCTGTTATTGA
AAGTAATGGTGAGGCTCAACAAATAAGTGTAATTGATAAACAAGGTCAATCGCATGATGTCACTTTACCTTCTAGCGTAT
TGAAAATGATGATTGAAGTGTTGACGCAATTGGGGCAGGGTAATTCTGTTAATATCACCCCCATCCATGCCGAAATGACC
ACGCAACAAGGCGCTGACTTTTTGAATATGTCACGGCCAACATTTATCAAGTTATTAGATTCACGTGAAATACCATTTAC
TCGAGTCGGTAATCGAAGAAAAGTGCCATTTTCTGCGGTGATAAAATATAAGCAGCAACTGGAAATCCAGCGTTTGTCAA
CACTTGATGAGCTTTCCAGATTAGATCAGGAATTGGGTATGGGTTACTAA