Detailed information of TA system TA027067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-RelB
• Location: 14424..14926
• Replicon: plasmid pSBAL11703
• Accession: NC_017578
• Organism: Shewanella baltica OS117

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A3DB69
• Locus tag: SBAL117_RS24315
• Protein ID: WP_011839997.1
• Coordinates: 14424..14678 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A3DB70
• Locus tag: SBAL117_RS24320
• Protein ID: WP_011839998.1
• Coordinates: 14675..14926 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL117_RS24270	9697..10197	-	501	WP_011839992.1	hypothetical protein	-
SBAL117_RS24275	10405..10881	+	477	WP_011839993.1	DUF417 family protein	-
SBAL117_RS24280	10979..11560	-	582	WP_011839994.1	DUF2913 family protein	-
SBAL117_RS24285	11557..11835	-	279	WP_011839995.1	hypothetical protein	-
SBAL117_RS25265	12394..12693	+	300	WP_014620443.1	hypothetical protein	-
SBAL117_RS24305	12653..13582	+	930	WP_014620444.1	integrase	-
SBAL117_RS24310	14134..14409	-	276	WP_148258346.1	hypothetical protein	-
SBAL117_RS24315	14424..14678	-	255	WP_011839997.1	Txe/YoeB family addiction module toxin	Toxin
SBAL117_RS24320	14675..14926	-	252	WP_011839998.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SBAL117_RS24325	15190..15639	+	450	WP_011839999.1	helix-turn-helix domain-containing protein	-
SBAL117_RS24330	15639..16208	+	570	WP_011840000.1	PIN domain-containing protein	-
SBAL117_RS24335	16271..17476	-	1206	WP_014620445.1	IS256-like element ISSod5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71173	71173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (1-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10165.67 Da
• Isoelectric Point: 9.8630

>T27067 WP_011839997.1 NC_017578:c14678-14424 [Shewanella baltica OS117]
MKILFSSNAWDDYLYWQQQDKKTLKRVNLLIKECQRAPFEGIGKPERLKENLSGYWSRRITDEHRLVYKASQDTLTIVAL
RYHY

Nucleotide

• Length: 255 bp

>T27067 NC_017578:c14678-14424 [Shewanella baltica OS117]
ATGAAAATTCTTTTTTCTTCCAACGCCTGGGATGACTACCTTTACTGGCAACAGCAGGATAAAAAAACGTTGAAACGAGT
TAATCTCCTGATTAAAGAATGCCAAAGGGCCCCATTTGAAGGTATTGGTAAACCGGAAAGATTAAAAGAAAATTTATCGG
GGTATTGGTCTCGCAGGATCACCGATGAACATCGATTAGTCTATAAGGCTAGTCAAGATACGTTGACGATCGTAGCGCTC
CGATATCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9487.57 Da
• Isoelectric Point: 4.7993

>AT27067 WP_011839998.1 NC_017578:c14926-14675 [Shewanella baltica OS117]
MAIYSFTEVRSNFKSICDTVVNDCEPVVIHRRGNENVVLFSESEFNSWKETLYLLSNPANAKRLLESIKQVNDGHASEHE
LSE

Nucleotide

• Length: 252 bp

>AT27067 NC_017578:c14926-14675 [Shewanella baltica OS117]
ATGGCTATTTACAGTTTCACAGAAGTTAGAAGTAACTTTAAGTCTATTTGCGACACTGTCGTCAATGATTGTGAGCCCGT
GGTAATACACCGTCGGGGTAACGAGAATGTAGTGTTATTTTCAGAATCTGAATTTAATAGTTGGAAGGAGACGCTATATT
TATTGTCGAACCCTGCTAATGCTAAACGTTTATTAGAATCCATAAAGCAAGTCAATGATGGACATGCTTCTGAGCATGAG
CTATCAGAATGA

Structures

Toxin

• AlphaFold DB: A3DB69

Antitoxin

• AlphaFold DB: A3DB70