Detailed information of TA system TA027066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc(toxin)
• Location: 5326..5882
• Replicon: plasmid pSBAL11703
• Accession: NC_017578
• Organism: Shewanella baltica OS117

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A3DB58
• Locus tag: SBAL117_RS24240
• Protein ID: WP_011839986.1
• Coordinates: 5326..5712 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A3DB59
• Locus tag: SBAL117_RS24245
• Protein ID: WP_011839987.1
• Coordinates: 5712..5882 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL117_RS24225	1012..3996	+	2985	WP_011846847.1	Tn3-like element ISSba14 family transposase	-
SBAL117_RS24230	4491..4829	-	339	WP_011839985.1	hypothetical protein	-
SBAL117_RS24235	4925..5245	-	321	WP_014620440.1	hypothetical protein	-
SBAL117_RS24240	5326..5712	-	387	WP_011839986.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
SBAL117_RS24245	5712..5882	-	171	WP_011839987.1	hypothetical protein	Antitoxin
SBAL117_RS24250	6016..6447	-	432	WP_011839988.1	DUF3577 domain-containing protein	-
SBAL117_RS24255	6752..8875	-	2124	WP_014620441.1	DNA topoisomerase III	-
SBAL117_RS24260	8885..9196	-	312	WP_011839990.1	hypothetical protein	-
SBAL117_RS24265	9199..9648	-	450	WP_011839991.1	single-stranded DNA-binding protein	-
SBAL117_RS24270	9697..10197	-	501	WP_011839992.1	hypothetical protein	-
SBAL117_RS24275	10405..10881	+	477	WP_011839993.1	DUF417 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71173	71173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (10-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14302.17 Da
• Isoelectric Point: 3.9061

>T27066 WP_011839986.1 NC_017578:c5712-5326 [Shewanella baltica OS117]
MNFIQFPAERVVEINQAILLNEPGFRGEYDIGRLSGALSRIDNAIAYEGLDDVFLIAAKYATSVAMAHAFSDANKRTGLA
LCLEYLSLNDYEINTEYDELSDAMVDLVVGTLSEIEFADVLYSMWRSM

Nucleotide

• Length: 387 bp

>T27066 NC_017578:c5712-5326 [Shewanella baltica OS117]
ATGAATTTCATTCAGTTTCCTGCTGAACGAGTCGTTGAAATCAATCAGGCCATTCTGTTGAATGAACCAGGTTTTCGCGG
TGAATATGATATCGGCAGATTGTCGGGTGCGTTATCGCGGATCGACAATGCTATTGCTTATGAAGGCTTGGATGATGTGT
TTTTAATTGCGGCTAAATATGCGACTTCAGTCGCTATGGCACACGCTTTTTCAGATGCAAACAAGCGCACAGGGCTTGCC
TTGTGTTTAGAGTATTTGTCACTCAATGATTACGAAATAAACACTGAGTATGATGAATTATCGGATGCAATGGTTGATTT
AGTTGTTGGTACACTAAGCGAAATCGAATTTGCAGATGTTCTGTATTCAATGTGGCGATCAATGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6390.54 Da
• Isoelectric Point: 10.7315

>AT27066 WP_011839987.1 NC_017578:c5882-5712 [Shewanella baltica OS117]
MLNETKKYGVNIVERPHVKANKKLDLTGGCGKQIVHSETKLVLRTHKNTFRKLADM

Nucleotide

• Length: 171 bp

>AT27066 NC_017578:c5882-5712 [Shewanella baltica OS117]
ATGTTAAATGAAACAAAGAAATACGGTGTAAACATCGTAGAGCGTCCACATGTGAAAGCCAATAAAAAGCTTGATTTGAC
TGGTGGTTGCGGTAAGCAGATTGTGCATAGCGAAACAAAACTTGTTTTACGAACGCATAAAAATACTTTTCGCAAACTGG
CTGATATGTAA

Structures

Toxin

• AlphaFold DB: A3DB58

Antitoxin

• AlphaFold DB: A3DB59