Detailed information of TA system TA027059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 838590..840207
• Replicon: plasmid megaplasmid
• Accession: NC_017575
• Organism: Ralstonia solanacearum Po82

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: F6GA13
• Locus tag: RSPO_RS19400
• Protein ID: WP_014619008.1
• Coordinates: 838590..839936 (-)
• Length: 449 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: RSPO_RS19405
• Protein ID: WP_042549221.1
• Coordinates: 839941..840207 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSPO_RS25840	833922..834068	+	147	WP_014619002.1	hypothetical protein	-
RSPO_RS19380	834216..834842	-	627	WP_014619003.1	FMN-dependent NADH-azoreductase	-
RSPO_RS19385	834981..835931	+	951	WP_039552434.1	LysR family transcriptional regulator	-
RSPO_RS19390	836024..837595	+	1572	WP_014619006.1	MFS transporter	-
RSPO_RS19395	837599..838495	-	897	WP_014619007.1	DUF2236 domain-containing protein	-
RSPO_RS19400	838590..839936	-	1347	WP_014619008.1	type II toxin-antitoxin system HipA family toxin	Toxin
RSPO_RS19405	839941..840207	-	267	WP_042549221.1	helix-turn-helix domain-containing protein	Antitoxin
RSPO_RS19410	840466..841317	+	852	WP_124994750.1	hypothetical protein	-
RSPO_RS19415	841333..841767	-	435	WP_042549879.1	PGDYG domain-containing protein	-
RSPO_RS19420	842024..842407	+	384	WP_014619012.1	VOC family protein	-
RSPO_RS19425	842446..843564	-	1119	WP_014619013.1	dienelactone hydrolase	-
RSPO_RS19430	843567..844403	-	837	WP_014619014.1	AraC family transcriptional regulator	-
RSPO_RS19435	844506..844982	-	477	WP_003265832.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / motA / cheW / cheB / cheY / adeG / acrB / fliI / fliM / flgG	1..1949172	1949172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (159-413)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-67)

Sequences

Toxin

Protein

• Length: 449 a.a.
• Molecular weight: 49543.83 Da
• Isoelectric Point: 7.8550

>T27059 WP_014619008.1 NC_017575:c839936-838590 [Ralstonia solanacearum Po82]
MGRRSHTRALGLWMNGTHVGRWELHPHQGDVLKYAASWVAAPEGRPLSLSLPFTPDNAPHKGPAVRAYFENLLPDSRDIR
ERIARRFQAGTTEAFDLLEQIGRDCIGALQVLPADMAPPATGDIDAEPLTEAQIAQTLRETVAPPPLGGMPEDDSHFRIS
IAGAQEKTAFLQIDGHWYRPRGATPTSHIFKLPMGLVGNMKLDLSDSVENEWLCALVLRAYGLPVAHCRPLVFEDQKVLA
VERFDRMWWDRGGKRLLRLPQEDMCQATATPPHLKYEADGGPGIDRIMGLLDGSAHREADRLTLFRSQVLFWMLCAPDGH
AKNFSLALRPGGAYALTPLYDVMSAYPLLGEGPGKLSPHRIRLAMAVRSKNAHWKMQDIQRRHWLGLGQRYGIVTSDGAD
AASIVDNLVARTPEVARTVRALLPEGFPQPLADRILQGLRHAADKLAG

Nucleotide

• Length: 1347 bp

>T27059 NC_017575:c839936-838590 [Ralstonia solanacearum Po82]
ATGGGGCGCCGGTCGCATACGCGGGCCCTCGGGCTCTGGATGAATGGCACCCATGTCGGCCGCTGGGAGCTGCACCCGCA
CCAGGGCGACGTGCTGAAGTACGCGGCATCCTGGGTGGCCGCGCCCGAGGGTCGGCCCCTGTCGCTGTCCCTGCCGTTCA
CGCCGGACAACGCGCCCCACAAGGGCCCGGCGGTCCGGGCGTATTTCGAGAACCTCCTGCCGGACAGCCGCGACATCCGC
GAGCGCATCGCGCGCCGTTTCCAGGCCGGGACAACCGAGGCGTTCGACCTGCTGGAGCAGATCGGCCGCGACTGCATCGG
CGCCCTGCAAGTGCTGCCGGCCGACATGGCCCCACCGGCCACGGGCGACATCGACGCGGAACCGCTGACCGAGGCGCAGA
TCGCGCAGACGCTGCGGGAGACCGTCGCCCCGCCCCCCTTGGGCGGCATGCCGGAAGACGATAGCCATTTCCGCATCTCG
ATCGCCGGCGCGCAGGAAAAGACGGCCTTCCTCCAGATCGACGGACACTGGTACCGCCCCCGGGGCGCGACGCCAACCAG
CCACATCTTCAAGCTGCCGATGGGGCTGGTCGGCAACATGAAGCTGGACCTGAGCGATTCGGTGGAAAACGAATGGCTGT
GCGCCCTGGTGCTGCGGGCGTATGGCCTGCCCGTCGCGCACTGCCGGCCGCTGGTGTTCGAAGACCAGAAGGTGCTCGCC
GTGGAGCGGTTCGACCGCATGTGGTGGGACCGCGGCGGCAAACGGCTCCTCCGCCTGCCGCAGGAAGACATGTGCCAGGC
CACGGCGACGCCCCCGCACCTGAAGTACGAGGCCGACGGCGGCCCCGGCATCGACCGCATCATGGGCCTGCTCGACGGCT
CCGCGCACCGGGAGGCCGACCGCCTGACGCTGTTCCGCAGCCAGGTGCTGTTCTGGATGCTGTGTGCGCCCGATGGCCAC
GCGAAGAACTTCAGCCTCGCGCTACGTCCCGGCGGCGCCTACGCGCTCACGCCGCTGTACGACGTGATGTCGGCCTACCC
GCTGCTGGGCGAGGGGCCGGGCAAGCTGTCGCCGCACCGGATCCGGCTGGCGATGGCCGTCCGCTCGAAGAATGCGCACT
GGAAAATGCAGGACATCCAGCGTCGCCACTGGCTGGGCCTGGGCCAACGCTACGGCATCGTCACGTCCGATGGCGCGGAT
GCCGCCTCCATCGTGGACAACCTGGTGGCCCGGACGCCCGAGGTGGCCCGTACCGTGCGCGCGCTGCTGCCGGAAGGCTT
CCCGCAGCCGCTGGCCGACCGCATTCTGCAGGGGCTGCGGCACGCTGCGGACAAACTCGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9636.14 Da
• Isoelectric Point: 10.9487

>AT27059 WP_042549221.1 NC_017575:c840207-839941 [Ralstonia solanacearum Po82]
MNEFIVRTPEQLPAILKGFRKQAGLSQAELAARMGVRQQTLSALERNAENVSAGRLMRLLSVLGVELVLRKPDAAGEPGR
PASDQPQW

Nucleotide

• Length: 267 bp

>AT27059 NC_017575:c840207-839941 [Ralstonia solanacearum Po82]
ATGAACGAGTTCATCGTACGCACCCCCGAGCAGTTGCCGGCGATCCTCAAGGGCTTCCGTAAACAGGCCGGGCTGAGCCA
GGCCGAACTGGCCGCCCGGATGGGCGTGCGCCAGCAAACCCTCTCAGCGCTGGAGCGCAACGCAGAAAACGTGAGCGCCG
GGCGGCTGATGCGCCTGCTGTCGGTGCTGGGCGTGGAGCTGGTGCTGCGCAAACCCGACGCCGCCGGCGAGCCCGGCCGC
CCGGCGTCCGACCAGCCGCAGTGGTGA

Structures

Toxin

• AlphaFold DB: F6GA13