Detailed information of TA system TA027057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 73377..73987
• Replicon: plasmid megaplasmid
• Accession: NC_017575
• Organism: Ralstonia solanacearum Po82

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F6G747
• Locus tag: RSPO_RS16395
• Protein ID: WP_014618391.1
• Coordinates: 73679..73987 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: RSPO_RS16390
• Protein ID: WP_014618390.1
• Coordinates: 73377..73682 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSPO_RS16375	69854..70642	-	789	WP_014618388.1	IS21-like element ISRso19 family helper ATPase IstB	-
RSPO_RS16380	70639..71655	-	1017	WP_039548595.1	IS21 family transposase	-
RSPO_RS16385	71851..73164	-	1314	WP_014618389.1	metabolite/H+ symporter	-
RSPO_RS16390	73377..73682	-	306	WP_014618390.1	putative addiction module antidote protein	Antitoxin
RSPO_RS16395	73679..73987	-	309	WP_014618391.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RSPO_RS16400	74063..75262	-	1200	WP_003262834.1	MFS transporter	-
RSPO_RS16405	76159..78876	+	2718	WP_014618393.1	type VI secretion system tip protein VgrG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / motA / cheW / cheB / cheY / adeG / acrB / fliI / fliM / flgG	1..1949172	1949172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11884.67 Da
• Isoelectric Point: 6.7111

>T27057 WP_014618391.1 NC_017575:c73987-73679 [Ralstonia solanacearum Po82]
MLTIRTTELFDDWFCSLQDKAAQRRIQVRIDRLQMAHPGDMKAVRDDIRELRIDHGPGYRLYFVQHGVVLIVLLCGGAKS
TQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 309 bp

>T27057 NC_017575:c73987-73679 [Ralstonia solanacearum Po82]
ATGCTGACGATCCGCACGACCGAACTCTTTGACGACTGGTTTTGCAGCCTCCAAGACAAGGCTGCGCAACGCCGCATCCA
GGTGCGCATCGACCGCCTGCAGATGGCTCACCCGGGCGACATGAAAGCGGTACGTGACGACATCCGTGAGCTGCGGATCG
ATCATGGGCCGGGCTACCGCTTGTACTTCGTACAGCATGGCGTCGTCCTGATCGTACTGCTCTGCGGCGGCGCCAAGTCG
ACGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11016.75 Da
• Isoelectric Point: 8.0667

>AT27057 WP_014618390.1 NC_017575:c73682-73377 [Ralstonia solanacearum Po82]
MTMAQRIIKTRPWDSAEHLRTEADMAAYLNACLEEAGDDPALITHALGVVARARGMTRLARETGITREGLYKALSEDGNP
SFATMLKVIRALGIRLHAVPA

Nucleotide

• Length: 306 bp

>AT27057 NC_017575:c73682-73377 [Ralstonia solanacearum Po82]
ATGACGATGGCCCAACGCATCATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATGGCCGC
CTATCTCAACGCCTGCCTGGAAGAAGCCGGGGACGACCCGGCGCTCATTACGCACGCACTGGGCGTGGTCGCGCGTGCGC
GCGGCATGACCCGGCTCGCCCGCGAGACCGGGATCACGCGCGAAGGGCTGTACAAGGCGCTTTCCGAGGACGGCAACCCG
AGCTTTGCGACCATGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: F6G747