Detailed information of TA system TA027055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2222647..2223232
• Replicon: chromosome
• Accession: NC_017574
• Organism: Ralstonia solanacearum Po82

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: F6G268
• Locus tag: RSPO_RS10095
• Protein ID: WP_014617297.1
• Coordinates: 2222647..2222955 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F6G269
• Locus tag: RSPO_RS10100
• Protein ID: WP_014617298.1
• Coordinates: 2222948..2223232 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSPO_RS10075	2218820..2219737	-	918	WP_014617293.1	sugar ABC transporter substrate-binding protein	-
RSPO_RS10080	2219947..2220999	-	1053	WP_039549673.1	LacI family DNA-binding transcriptional regulator	-
RSPO_RS10085	2221134..2222291	+	1158	WP_052307006.1	fatty acid desaturase family protein	-
RSPO_RS10090	2222257..2222568	+	312	WP_014617296.1	Rieske 2Fe-2S domain-containing protein	-
RSPO_RS10095	2222647..2222955	+	309	WP_014617297.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RSPO_RS10100	2222948..2223232	+	285	WP_014617298.1	putative addiction module antidote protein	Antitoxin
RSPO_RS10105	2223246..2224091	-	846	WP_014617299.1	TIM barrel protein	-
RSPO_RS10110	2224088..2225125	-	1038	WP_014617300.1	hypothetical protein	-
RSPO_RS10115	2225122..2226411	-	1290	WP_014617301.1	FAD-dependent oxidoreductase	-
RSPO_RS10120	2226579..2227496	+	918	WP_014617302.1	MurR/RpiR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11361.99 Da
• Isoelectric Point: 9.8179

>T27055 WP_014617297.1 NC_017574:2222647-2222955 [Ralstonia solanacearum Po82]
MHTLIRSDTFDQWLEKLSDKRVKARITARLVSASFGNVGDCKPVGEGISEMRIDVGAGYRVYYTRQGTVVYILLTGGSKA
TQAQDIKQAIRMARELKESGHD

Nucleotide

• Length: 309 bp

>T27055 NC_017574:2222647-2222955 [Ralstonia solanacearum Po82]
ATGCATACGCTGATCCGCTCCGACACGTTCGACCAGTGGCTTGAAAAGCTGAGCGACAAGCGCGTCAAGGCGCGGATCAC
CGCCCGGCTGGTCAGCGCATCATTCGGGAATGTTGGCGACTGCAAGCCCGTGGGGGAAGGCATCAGCGAAATGCGGATCG
ATGTGGGCGCCGGGTATCGCGTCTACTACACGCGACAAGGTACCGTCGTCTACATCCTGCTCACGGGCGGCAGCAAAGCA
ACCCAAGCCCAGGATATCAAGCAAGCCATCCGGATGGCACGCGAGTTGAAGGAGTCAGGCCATGACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 9905.28 Da
• Isoelectric Point: 4.7361

>AT27055 WP_014617298.1 NC_017574:2222948-2223232 [Ralstonia solanacearum Po82]
MTETYAPFDAAAYLDSEETIAEFLAAAMEDPNPDVFLAALGAVARARGIAKVAADAGLGRESLYKAISPGAHPRYETVQK
IVRALGVRLNVTPA

Nucleotide

• Length: 285 bp

>AT27055 NC_017574:2222948-2223232 [Ralstonia solanacearum Po82]
ATGACTGAGACCTACGCACCGTTTGACGCGGCGGCCTATCTCGATAGCGAAGAGACCATCGCCGAATTTCTGGCCGCCGC
CATGGAAGACCCGAATCCGGACGTCTTCCTGGCTGCGCTCGGCGCCGTCGCGCGCGCCCGCGGCATTGCCAAGGTGGCCG
CGGACGCGGGCCTGGGGCGTGAAAGCCTGTACAAGGCCATCAGCCCGGGCGCACATCCACGCTACGAAACCGTCCAGAAA
ATCGTGCGCGCATTGGGCGTCAGGTTGAACGTGACGCCCGCTTGA

Structures

Toxin

• AlphaFold DB: F6G268

Antitoxin

• AlphaFold DB: F6G269