Detailed information of TA system TA027054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 459121..459754
• Replicon: chromosome
• Accession: NC_017574
• Organism: Ralstonia solanacearum Po82

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F6FXL1
• Locus tag: RSPO_RS02030
• Protein ID: WP_014615899.1
• Coordinates: 459341..459754 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F6FXL0
• Locus tag: RSPO_RS02025
• Protein ID: WP_003263271.1
• Coordinates: 459121..459354 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RSPO_RS02010	454695..455984	-	1290	WP_003263268.1	MFS transporter	-
RSPO_RS02015	455992..457260	-	1269	WP_124994693.1	AGE family epimerase/isomerase	-
RSPO_RS02020	457456..459063	+	1608	WP_039567295.1	glucan biosynthesis protein	-
RSPO_RS02025	459121..459354	+	234	WP_003263271.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
RSPO_RS02030	459341..459754	+	414	WP_014615899.1	PIN domain-containing protein	Toxin
RSPO_RS02035	459729..460355	-	627	WP_014615900.1	DUF1415 family protein	-
RSPO_RS02040	460566..460940	+	375	WP_014615901.1	hypothetical protein	-
RSPO_RS02045	461015..462511	-	1497	WP_014615902.1	glycerol kinase GlpK	-
RSPO_RS02050	462683..464419	-	1737	WP_003266149.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-40)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14919.23 Da
• Isoelectric Point: 5.9701

>T27054 WP_014615899.1 NC_017574:459341-459754 [Ralstonia solanacearum Po82]
MPAAEAFFDSNIVLYLLSADAAKADAAEALLMAGGVVSVQVLNETTHVMRRKLAMPWHAIEAVQEAVRAQCRVEPLTVET
HDLGRHLAQRYGLSVYDALIVAAASLAGCRVLYSEDMQHGLVIEQRLRVVNPFFASA

Nucleotide

• Length: 414 bp

>T27054 NC_017574:459341-459754 [Ralstonia solanacearum Po82]
ATGCCCGCGGCTGAGGCGTTTTTCGACAGCAACATCGTGCTGTACCTGCTGTCGGCCGATGCCGCCAAGGCCGATGCGGC
GGAAGCGCTACTGATGGCCGGCGGCGTCGTCAGCGTGCAGGTGCTCAACGAAACGACGCACGTGATGCGCCGCAAGCTCG
CCATGCCGTGGCACGCGATCGAAGCGGTGCAGGAAGCCGTACGCGCGCAATGCCGGGTCGAGCCGCTGACGGTCGAAACC
CATGATCTCGGGCGGCATCTTGCGCAGCGCTATGGATTGTCGGTCTATGACGCGCTGATCGTCGCCGCAGCGTCGCTGGC
CGGTTGCCGCGTCCTGTATTCCGAGGACATGCAGCACGGCCTGGTGATCGAGCAGCGTCTGCGCGTCGTCAATCCTTTTT
TCGCGTCGGCGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8664.82 Da
• Isoelectric Point: 8.7288

>AT27054 WP_003263271.1 NC_017574:459121-459354 [Ralstonia solanacearum Po82]
MQVSKWGNSLAIRLPAAVVEALGLHEGDDVEVVVAGERSFGVRRKRSARELFERLHQYRGKLPADFIFERDDANARG

Nucleotide

• Length: 234 bp

>AT27054 NC_017574:459121-459354 [Ralstonia solanacearum Po82]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCCATCCGGCTGCCCGCAGCCGTTGTGGAGGCGCTTGGACTGCACGAAGG
CGACGACGTCGAGGTGGTGGTGGCGGGCGAACGCTCGTTTGGTGTGCGCCGCAAGCGTTCCGCGCGTGAGTTGTTTGAGC
GGCTCCACCAATACCGCGGCAAGCTGCCCGCCGACTTCATCTTCGAGCGCGACGACGCCAATGCCCGCGGCTGA

Structures

Toxin

• AlphaFold DB: F6FXL1

Antitoxin

• AlphaFold DB: F6FXL0