Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3059893..3060463 Replicon chromosome
Accession NZ_CP117298
Organism Mycobacterium tuberculosis variant bovis strain OLF-AH-2022-TB-0122

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag PQK93_RS14520 Protein ID WP_003414166.1
Coordinates 3059893..3060249 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag PQK93_RS14525 Protein ID WP_003901465.1
Coordinates 3060233..3060463 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQK93_RS14500 (PQK93_14500) 3055342..3057030 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
PQK93_RS14505 (PQK93_14505) 3057034..3057360 - 327 WP_003414157.1 hypothetical protein -
PQK93_RS14510 (PQK93_14510) 3057533..3058123 + 591 WP_031656198.1 DUF3558 family protein -
PQK93_RS14515 (PQK93_14515) 3058142..3059791 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
PQK93_RS14520 (PQK93_14520) 3059893..3060249 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
PQK93_RS14525 (PQK93_14525) 3060233..3060463 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
PQK93_RS14530 (PQK93_14530) 3060506..3061549 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
PQK93_RS14535 (PQK93_14535) 3061548..3062015 + 468 WP_003414177.1 DUF1778 domain-containing protein -
PQK93_RS14540 (PQK93_14540) 3062191..3062445 - 255 WP_003917684.1 hypothetical protein -
PQK93_RS14545 (PQK93_14545) 3062593..3062997 + 405 WP_003414181.1 hypothetical protein -
PQK93_RS14550 (PQK93_14550) 3062994..3063185 + 192 WP_003414184.1 hypothetical protein -
PQK93_RS14555 (PQK93_14555) 3063402..3063662 + 261 Protein_2872 transposase -
PQK93_RS14560 (PQK93_14560) 3064772..3065029 + 258 WP_003899489.1 hypothetical protein -
PQK93_RS14565 (PQK93_14565) 3065134..3065445 + 312 WP_010950777.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T270365 WP_003414166.1 NZ_CP117298:c3060249-3059893 [Mycobacterium tuberculosis variant bovis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT270365 WP_003901465.1 NZ_CP117298:c3060463-3060233 [Mycobacterium tuberculosis variant bovis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References