Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3019895..3020574 Replicon chromosome
Accession NZ_CP117298
Organism Mycobacterium tuberculosis variant bovis strain OLF-AH-2022-TB-0122

Toxin (Protein)


Gene name vapC Uniprot ID P9WF90
Locus tag PQK93_RS14290 Protein ID WP_003414059.1
Coordinates 3019895..3020311 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag PQK93_RS14295 Protein ID WP_003414061.1
Coordinates 3020308..3020574 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQK93_RS14270 (PQK93_14270) 3015947..3016849 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
PQK93_RS14275 (PQK93_14275) 3016918..3017670 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
PQK93_RS14280 (PQK93_14280) 3017914..3018189 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
PQK93_RS14285 (PQK93_14285) 3018186..3019808 - 1623 WP_003414057.1 class I SAM-dependent DNA methyltransferase -
PQK93_RS14290 (PQK93_14290) 3019895..3020311 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 Toxin
PQK93_RS14295 (PQK93_14295) 3020308..3020574 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
PQK93_RS14300 (PQK93_14300) 3020600..3020995 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin -
PQK93_RS14305 (PQK93_14305) 3020992..3021261 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
PQK93_RS14310 (PQK93_14310) 3021271..3022365 - 1095 WP_105537259.1 restriction endonuclease subunit S -
PQK93_RS14315 (PQK93_14315) 3022362..3022781 - 420 Protein_2824 winged helix-turn-helix domain-containing protein -
PQK93_RS14320 (PQK93_14320) 3022780..3022854 + 75 Protein_2825 hypothetical protein -
PQK93_RS14325 (PQK93_14325) 3022855..3023334 - 480 WP_003414073.1 dihydrofolate reductase -
PQK93_RS14330 (PQK93_14330) 3023405..3024205 - 801 WP_003911953.1 thymidylate synthase -
PQK93_RS14335 (PQK93_14335) 3024361..3025098 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-123)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T270363 WP_003414059.1 NZ_CP117298:c3020311-3019895 [Mycobacterium tuberculosis variant bovis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT270363 WP_003414061.1 NZ_CP117298:c3020574-3020308 [Mycobacterium tuberculosis variant bovis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5SV2

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References