Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2206445..2207031 Replicon chromosome
Accession NZ_CP117298
Organism Mycobacterium tuberculosis variant bovis strain OLF-AH-2022-TB-0122

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag PQK93_RS10325 Protein ID WP_003410010.1
Coordinates 2206445..2206789 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag PQK93_RS10330 Protein ID WP_003410014.1
Coordinates 2206783..2207031 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQK93_RS10290 (PQK93_10290) 2202150..2202749 + 600 WP_003409989.1 L-lysine exporter -
PQK93_RS10295 (PQK93_10295) 2203165..2203593 + 429 WP_003409992.1 cellulose-binding protein -
PQK93_RS10300 (PQK93_10300) 2203819..2204358 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
PQK93_RS10305 (PQK93_10305) 2204878..2205438 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
PQK93_RS10310 (PQK93_10310) 2205435..2205776 - 342 WP_003410003.1 DUF2384 domain-containing protein -
PQK93_RS10315 (PQK93_10315) 2205862..2206119 + 258 WP_003410006.1 hypothetical protein -
PQK93_RS10320 (PQK93_10320) 2206020..2206355 - 336 WP_003410009.1 dehydrogenase -
PQK93_RS10325 (PQK93_10325) 2206445..2206789 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
PQK93_RS10330 (PQK93_10330) 2206783..2207031 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
PQK93_RS10335 (PQK93_10335) 2207131..2209446 - 2316 WP_003899120.1 cation transporter ATPase CptG -
PQK93_RS10340 (PQK93_10340) 2209443..2209715 - 273 WP_003410017.1 DUF1490 family protein -
PQK93_RS10345 (PQK93_10345) 2209768..2210124 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
PQK93_RS10350 (PQK93_10350) 2210281..2211048 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T270351 WP_003410010.1 NZ_CP117298:c2206789-2206445 [Mycobacterium tuberculosis variant bovis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT270351 WP_003410014.1 NZ_CP117298:c2207031-2206783 [Mycobacterium tuberculosis variant bovis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References