Detailed information of TA system TA027030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /ElaA-DUF1778
• Location: 57551..58334
• Replicon: plasmid pSBAL17501
• Accession: NC_017570
• Organism: Shewanella baltica BA175

Toxin (Protein)

• Gene name: -
• Uniprot ID: B8EFS1
• Locus tag: SBAL175_RS22100
• Protein ID: WP_011979514.1
• Coordinates: 57551..58060 (-)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B8EFS2
• Locus tag: SBAL175_RS22105
• Protein ID: WP_011979513.1
• Coordinates: 58041..58334 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBAL175_RS22080	52861..53859	-	999	WP_011979516.1	DUF1738 domain-containing protein	-
SBAL175_RS22085	54402..55595	+	1194	WP_014615250.1	IS4 family transposase	-
SBAL175_RS22090	55830..56633	-	804	WP_014615251.1	hypothetical protein	-
SBAL175_RS23185	56831..57031	+	201	Protein_45	IS4 family transposase	-
SBAL175_RS22095	57211..57438	-	228	WP_011979515.1	hypothetical protein	-
SBAL175_RS22100	57551..58060	-	510	WP_011979514.1	GNAT family N-acetyltransferase	Toxin
SBAL175_RS22105	58041..58334	-	294	WP_011979513.1	DUF1778 domain-containing protein	Antitoxin
SBAL175_RS22110	58704..59105	-	402	WP_012589050.1	hypothetical protein	-
SBAL175_RS22115	59107..59730	-	624	WP_014615252.1	hypothetical protein	-
SBAL175_RS22120	60402..60623	-	222	WP_014615254.1	hypothetical protein	-
SBAL175_RS22125	60654..61142	-	489	WP_014615255.1	hypothetical protein	-
SBAL175_RS22130	61144..61599	-	456	WP_014615256.1	type II toxin-antitoxin system HicB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..72392	72392
	-	flank	IS/Tn	-	-	54402..55595	1193

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (80-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-89)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 19129.15 Da
• Isoelectric Point: 8.3399

>T27030 WP_011979514.1 NC_017570:c58060-57551 [Shewanella baltica BA175]
MKITQFDKNEHDRKSFDCGNTELNNYLKCISGQHEKKDISRTYVLTSELNPSHIKGYYSIAVAQVSLCELPTEIAKKYPS
EITCSLIGRMAVNHTCQRQGLGSILLIDAIKRIIQASSAIPIPMIIVQAKNEDARRFYSEFGFVSFPDNYLKLMMTLAHA
KAMLKEAEH

Nucleotide

• Length: 510 bp

>T27030 NC_017570:c58060-57551 [Shewanella baltica BA175]
ATGAAAATTACCCAATTTGATAAAAATGAGCATGACCGTAAGAGTTTTGATTGTGGGAATACAGAGCTAAACAATTACCT
GAAATGCATTTCAGGTCAGCATGAAAAGAAAGATATTTCAAGAACATATGTTCTTACTTCAGAGTTAAATCCTTCACACA
TAAAAGGTTATTACTCTATTGCGGTAGCTCAGGTATCACTTTGTGAGTTACCAACCGAAATAGCTAAAAAATATCCATCT
GAAATAACGTGTAGCTTGATAGGTCGAATGGCTGTTAACCATACCTGTCAAAGACAAGGGCTTGGCTCAATATTGCTTAT
AGACGCAATCAAACGAATTATCCAAGCTTCCAGTGCTATCCCCATCCCTATGATTATTGTTCAAGCAAAGAACGAAGATG
CTAGACGTTTTTATAGTGAGTTTGGTTTTGTATCCTTTCCTGATAATTATTTGAAACTCATGATGACACTGGCCCATGCT
AAAGCGATGTTGAAAGAAGCCGAACATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10811.39 Da
• Isoelectric Point: 10.0024

>AT27030 WP_011979513.1 NC_017570:c58334-58041 [Shewanella baltica BA175]
MKSMTNERITTRVSIESKELLEKAAALAGYASINSFIANTMISEAKRLITQEHFLVLSKNDALAFVNALDAPVKSNSRFL
NAARKHKETVNNENYPI

Nucleotide

• Length: 294 bp

>AT27030 NC_017570:c58334-58041 [Shewanella baltica BA175]
ATGAAAAGTATGACAAATGAACGCATTACCACTCGGGTAAGCATTGAGTCAAAAGAACTATTAGAGAAAGCGGCTGCGCT
CGCTGGTTATGCCAGTATTAACAGTTTTATTGCGAATACAATGATTTCAGAAGCGAAAAGATTGATCACACAAGAGCATT
TTCTGGTGTTATCAAAAAATGATGCTTTAGCTTTTGTGAACGCTTTAGATGCACCCGTTAAAAGTAACAGTCGGTTTCTT
AATGCCGCGCGTAAGCATAAGGAAACGGTAAACAATGAAAATTACCCAATTTGA

Structures

Toxin

• AlphaFold DB: A9L6I1

Antitoxin

• AlphaFold DB: A0A380BY85