Detailed information of TA system TA027025

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 31091..31625
• Replicon: plasmid pYV03
• Accession: NC_017565
• Organism: Yersinia enterocolitica subsp. palearctica Y11

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A0H3NX04
• Locus tag: Y11_RS20880
• Protein ID: WP_014609469.1
• Coordinates: 31326..31625 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P0C2N3
• Locus tag: Y11_RS20875
• Protein ID: WP_005176524.1
• Coordinates: 31091..31333 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Y11_RS20855	26715..26933	+	219	WP_013749479.1	hypothetical protein	-
Y11_RS20860	26994..27464	-	471	WP_010891240.1	hypothetical protein	-
Y11_RS20865	28080..29246	+	1167	WP_010891239.1	plasmid-partitioning protein SopA	-
Y11_RS20870	29243..30208	+	966	WP_014609466.1	ParB/RepB/Spo0J family plasmid partition protein	-
Y11_RS22585	30633..30925	+	293	Protein_31	IS6 family transposase	-
Y11_RS20875	31091..31333	+	243	WP_005176524.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
Y11_RS20880	31326..31625	+	300	WP_014609469.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Y11_RS20885	31765..32424	-	660	WP_010891237.1	type III secretion system effector GTPase activator YopE	-
Y11_RS20890	32617..33009	+	393	WP_010891236.1	type III secretion system chaperone SycE/YerA	-
Y11_RS22590	33266..34418	+	1153	Protein_36	IS3 family transposase	-
Y11_RS20905	34622..35047	+	426	WP_010891235.1	type III secretion system chaperone SycH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yopO/ypkA / yopJ/yopP / yopK/yopQ / yopT / sycT / yadA / yopE / sycE/yerA / sycH / yopH / lcrQ/yscM / yscL / yscK / ylpB/yscJ / lcrO/yscI / yopR/yscH/lcrP / yscG / yscF / yscE / yscD / yscC / yscB / virG/yscW / yscU / yscT / yscS / yscR / yscQ / yscO / yscN / lcrE/yopN / tyeA / sycN / yscX / yscY / yscV/lcrD / lcrR / lcrG / lcrV / sycD/lcrH / yopB / yopD / yopM	1..72460	72460
	-	flank	IS/Tn	-	-	33792..34295	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11664.24 Da
• Isoelectric Point: 5.1053

>T27025 WP_014609469.1 NC_017565:31326-31625 [Yersinia enterocolitica subsp. palearctica Y11]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNLELGAECNWICSDMRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDAQDFPEYE

Nucleotide

• Length: 300 bp

>T27025 NC_017565:31326-31625 [Yersinia enterocolitica subsp. palearctica Y11]
GTGTATAAACTATCTGAACTGGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCTAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGACATGCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGCTCAGGATTTTCCAGAGTATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8856.15 Da
• Isoelectric Point: 6.7232

>AT27025 WP_005176524.1 NC_017565:31091-31333 [Yersinia enterocolitica subsp. palearctica Y11]
MARVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNAPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT27025 NC_017565:31091-31333 [Yersinia enterocolitica subsp. palearctica Y11]
ATGGCTCGCGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAACGCACCTGTGAGCCATCGCTTAATGGATGAAATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: A0A0H3NX04

Antitoxin

• AlphaFold DB: A1JUB1