Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4337501..4338077 Replicon chromosome
Accession NZ_CP117235
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag PQQ28_RS21370 Protein ID WP_001295743.1
Coordinates 4337790..4338077 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag PQQ28_RS21365 Protein ID WP_000063148.1
Coordinates 4337501..4337803 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ28_RS21350 (4334097) 4334097..4336247 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PQQ28_RS21355 (4336297) 4336297..4336500 + 204 WP_000467859.1 YbdD/YjiX family protein -
PQQ28_RS21360 (4336511) 4336511..4337467 + 957 WP_001295745.1 GTPase -
PQQ28_RS21365 (4337501) 4337501..4337803 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
PQQ28_RS21370 (4337790) 4337790..4338077 - 288 WP_001295743.1 BrnT family toxin Toxin
PQQ28_RS21375 (4338274) 4338274..4339440 + 1167 WP_000800831.1 restriction endonuclease -
PQQ28_RS21380 (4339504) 4339504..4341123 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
PQQ28_RS21385 (4341113) 4341113..4342417 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T270142 WP_001295743.1 NZ_CP117235:c4338077-4337790 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT270142 WP_000063148.1 NZ_CP117235:c4337803-4337501 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References