Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2557250..2558838 Replicon chromosome
Accession NZ_CP117235
Organism Escherichia coli strain ATCC 25922

Toxin (Protein)


Gene name hipA Uniprot ID A0A1L1KDK2
Locus tag PQQ28_RS12600 Protein ID WP_001125431.1
Coordinates 2557516..2558838 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V0VAH2
Locus tag PQQ28_RS12595 Protein ID WP_001304368.1
Coordinates 2557250..2557516 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ28_RS12580 (2553618) 2553618..2555069 + 1452 WP_000854660.1 tagaturonate reductase -
PQQ28_RS12585 (2555266) 2555266..2556180 + 915 WP_001443133.1 bestrophin family protein -
PQQ28_RS12590 (2556184) 2556184..2556942 - 759 WP_001286540.1 trans-aconitate 2-methyltransferase -
PQQ28_RS12595 (2557250) 2557250..2557516 + 267 WP_001304368.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
PQQ28_RS12600 (2557516) 2557516..2558838 + 1323 WP_001125431.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
PQQ28_RS12605 (2559148) 2559148..2559555 - 408 Protein_2395 acetylxylan esterase -
PQQ28_RS12610 (2559558) 2559558..2560139 - 582 Protein_2396 LysR family transcriptional regulator -
PQQ28_RS12615 (2560288) 2560288..2561070 + 783 WP_001221834.1 SDR family oxidoreductase -
PQQ28_RS12620 (2561314) 2561314..2561421 - 108 Protein_2398 hypothetical protein -
PQQ28_RS12625 (2561846) 2561846..2562409 + 564 WP_001327671.1 type 1 fimbrial major subunit FimA -
PQQ28_RS12630 (2562771) 2562771..2563481 + 711 WP_001195181.1 fimbria/pilus periplasmic chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(14-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49310.02 Da        Isoelectric Point: 8.6972

>T270134 WP_001125431.1 NZ_CP117235:2557516-2558838 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVKRFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKNSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9957.31 Da        Isoelectric Point: 4.9632

>AT270134 WP_001304368.1 NZ_CP117235:2557250-2557516 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
AEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L1KDK2

Antitoxin

Source ID Structure
AlphaFold DB V0VAH2

References