Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4750879..4751689 Replicon chromosome
Accession NZ_CP117227
Organism Klebsiella pneumoniae strain ATCC BAA2146

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag PQQ24_RS25620 Protein ID WP_002887280.1
Coordinates 4750879..4751412 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag PQQ24_RS25625 Protein ID WP_002887278.1
Coordinates 4751423..4751689 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ24_RS25615 (PQQ24_25615) 4749710..4750831 + 1122 WP_002887282.1 cupin domain-containing protein -
PQQ24_RS25620 (PQQ24_25620) 4750879..4751412 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
PQQ24_RS25625 (PQQ24_25625) 4751423..4751689 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
PQQ24_RS25630 (PQQ24_25630) 4751792..4753225 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
PQQ24_RS25635 (PQQ24_25635) 4753215..4753898 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
PQQ24_RS25640 (PQQ24_25640) 4754070..4755455 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
PQQ24_RS25645 (PQQ24_25645) 4755473..4755817 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T270106 WP_002887280.1 NZ_CP117227:c4751412-4750879 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT270106 WP_002887278.1 NZ_CP117227:c4751689-4751423 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References