Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 58714..59450 Replicon plasmid pATCCBAA2146_3
Accession NZ_CP117225
Organism Klebsiella pneumoniae strain ATCC BAA2146

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag PQQ24_RS01125 Protein ID WP_003026803.1
Coordinates 58968..59450 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag PQQ24_RS01120 Protein ID WP_003026799.1
Coordinates 58714..58980 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PQQ24_RS01095 (PQQ24_01095) 53805..54152 + 348 WP_000612626.1 IS66 family insertion sequence element accessory protein TnpB -
PQQ24_RS01100 (PQQ24_01100) 54201..55739 + 1539 WP_004201219.1 IS66-like element ISKpn24 family transposase -
PQQ24_RS01105 (PQQ24_01105) 56638..56946 - 309 WP_017896554.1 hypothetical protein -
PQQ24_RS01110 (PQQ24_01110) 57440..57988 + 549 WP_001567366.1 thioredoxin fold domain-containing protein -
PQQ24_RS01115 (PQQ24_01115) 58035..58469 + 435 WP_001567367.1 cell envelope integrity protein TolA -
PQQ24_RS01120 (PQQ24_01120) 58714..58980 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
PQQ24_RS01125 (PQQ24_01125) 58968..59450 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
PQQ24_RS01130 (PQQ24_01130) 59651..61054 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
PQQ24_RS01135 (PQQ24_01135) 61083..61715 - 633 WP_001567369.1 hypothetical protein -
PQQ24_RS01140 (PQQ24_01140) 61941..62132 + 192 Protein_61 helix-turn-helix domain-containing protein -
PQQ24_RS01145 (PQQ24_01145) 62333..64096 + 1764 WP_004200842.1 DUF262 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / mph(A) - 1..117755 117755
- inside IScluster/Tn - - 53404..61054 7650


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T270094 WP_003026803.1 NZ_CP117225:58968-59450 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT270094 WP_003026799.1 NZ_CP117225:58714-58980 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References