Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1291780..1292697 Replicon chromosome
Accession NZ_CP117197
Organism Bacillus velezensis strain 3ZT

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag PO847_RS06815 Protein ID WP_007407256.1
Coordinates 1291951..1292697 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag PO847_RS06810 Protein ID WP_003154807.1
Coordinates 1291780..1291950 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO847_RS06770 (1287013) 1287013..1288635 + 1623 WP_022553476.1 pyocin knob domain-containing protein -
PO847_RS06775 (1288648) 1288648..1289019 + 372 WP_022553475.1 XkdW family protein -
PO847_RS06780 (1289024) 1289024..1289221 + 198 WP_007610833.1 XkdX family protein -
PO847_RS06785 (1289278) 1289278..1290039 + 762 WP_022553474.1 hypothetical protein -
PO847_RS06790 (1290091) 1290091..1290354 + 264 WP_022553473.1 hemolysin XhlA family protein -
PO847_RS06795 (1290368) 1290368..1290631 + 264 WP_003154813.1 phage holin -
PO847_RS06800 (1290645) 1290645..1291523 + 879 WP_007407257.1 N-acetylmuramoyl-L-alanine amidase -
PO847_RS06805 (1291558) 1291558..1291683 - 126 WP_003154809.1 hypothetical protein -
PO847_RS06810 (1291780) 1291780..1291950 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
PO847_RS06815 (1291951) 1291951..1292697 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
PO847_RS06820 (1292802) 1292802..1293800 - 999 WP_003154805.1 inorganic phosphate transporter -
PO847_RS06825 (1293813) 1293813..1294430 - 618 WP_003154804.1 DUF47 domain-containing protein -
PO847_RS06830 (1294716) 1294716..1296032 - 1317 WP_007610842.1 amino acid permease -
PO847_RS06835 (1296354) 1296354..1297304 + 951 WP_022553472.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1258070..1339197 81127


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T270064 WP_007407256.1 NZ_CP117197:c1292697-1291951 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT270064 WP_003154807.1 NZ_CP117197:c1291950-1291780 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References