Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485980..486617 Replicon chromosome
Accession NZ_CP117197
Organism Bacillus velezensis strain 3ZT

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PO847_RS02465 Protein ID WP_003156187.1
Coordinates 486267..486617 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PO847_RS02460 Protein ID WP_003156188.1
Coordinates 485980..486261 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO847_RS02440 (482345) 482345..482944 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PO847_RS02445 (483037) 483037..483402 + 366 WP_022552632.1 holo-ACP synthase -
PO847_RS02450 (483567) 483567..484574 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PO847_RS02455 (484691) 484691..485860 + 1170 WP_022552633.1 alanine racemase -
PO847_RS02460 (485980) 485980..486261 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PO847_RS02465 (486267) 486267..486617 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PO847_RS02470 (486735) 486735..487556 + 822 WP_003156182.1 STAS domain-containing protein -
PO847_RS02475 (487561) 487561..487926 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
PO847_RS02480 (487929) 487929..488330 + 402 WP_003156178.1 anti-sigma regulatory factor -
PO847_RS02485 (488342) 488342..489349 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
PO847_RS02490 (489413) 489413..489742 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PO847_RS02495 (489739) 489739..490221 + 483 WP_007609591.1 anti-sigma B factor RsbW -
PO847_RS02500 (490187) 490187..490975 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PO847_RS02505 (490975) 490975..491577 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T270063 WP_003156187.1 NZ_CP117197:486267-486617 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT270063 WP_003156188.1 NZ_CP117197:485980-486261 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References