Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 485976..486613 Replicon chromosome
Accession NZ_CP117182
Organism Bacillus velezensis strain Lwp6

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag PO767_RS02465 Protein ID WP_003156187.1
Coordinates 486263..486613 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag PO767_RS02460 Protein ID WP_003156188.1
Coordinates 485976..486257 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PO767_RS02440 (482341) 482341..482940 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
PO767_RS02445 (483033) 483033..483398 + 366 WP_022552632.1 holo-ACP synthase -
PO767_RS02450 (483563) 483563..484570 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
PO767_RS02455 (484687) 484687..485856 + 1170 WP_022552633.1 alanine racemase -
PO767_RS02460 (485976) 485976..486257 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
PO767_RS02465 (486263) 486263..486613 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
PO767_RS02470 (486731) 486731..487552 + 822 WP_003156182.1 STAS domain-containing protein -
PO767_RS02475 (487557) 487557..487922 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
PO767_RS02480 (487925) 487925..488326 + 402 WP_003156178.1 anti-sigma regulatory factor -
PO767_RS02485 (488338) 488338..489345 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
PO767_RS02490 (489409) 489409..489738 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
PO767_RS02495 (489735) 489735..490217 + 483 WP_007609591.1 anti-sigma B factor RsbW -
PO767_RS02500 (490183) 490183..490971 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
PO767_RS02505 (490971) 490971..491573 + 603 WP_007410234.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T270037 WP_003156187.1 NZ_CP117182:486263-486613 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT270037 WP_003156188.1 NZ_CP117182:485976-486257 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References