Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4507653..4508150 Replicon chromosome
Accession NZ_CP117053
Organism Escherichia coli strain MLI106K4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL407_RS23070 Protein ID WP_001173343.1
Coordinates 4507653..4507826 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL407_RS23075 Protein ID WP_000398619.1
Coordinates 4507854..4508150 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL407_RS23060 (4504323) 4504323..4505780 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
NL407_RS23065 (4506017) 4506017..4507534 + 1518 WP_001295074.1 lysine--tRNA ligase -
NL407_RS23070 (4507653) 4507653..4507826 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL407_RS23075 (4507854) 4507854..4508150 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL407_RS23080 (4508377) 4508377..4508649 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
NL407_RS23085 (4508661) 4508661..4508891 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NL407_RS23090 (4509072) 4509072..4510703 + 1632 WP_001216477.1 sensor histidine kinase -
NL407_RS23095 (4510700) 4510700..4511419 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269976 WP_001173343.1 NZ_CP117053:c4507826-4507653 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269976 WP_000398619.1 NZ_CP117053:c4508150-4507854 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References