Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 834237..834891 Replicon chromosome
Accession NZ_CP117053
Organism Escherichia coli strain MLI106K4

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NL407_RS05240 Protein ID WP_000244781.1
Coordinates 834484..834891 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL407_RS05235 Protein ID WP_000354046.1
Coordinates 834237..834503 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL407_RS05215 (830325) 830325..831758 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
NL407_RS05220 (831803) 831803..832114 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
NL407_RS05225 (832278) 832278..832937 + 660 WP_000250269.1 hemolysin III family protein -
NL407_RS05230 (833014) 833014..833994 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
NL407_RS05235 (834237) 834237..834503 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL407_RS05240 (834484) 834484..834891 + 408 WP_000244781.1 protein YgfX Toxin
NL407_RS05245 (834931) 834931..835452 - 522 WP_001521233.1 flavodoxin FldB -
NL407_RS05250 (835564) 835564..836460 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NL407_RS05255 (836485) 836485..837195 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL407_RS05260 (837201) 837201..838934 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T269955 WP_000244781.1 NZ_CP117053:834484-834891 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269955 WP_000354046.1 NZ_CP117053:834237-834503 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References