Detailed information of TA system TA026993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1884554..1885144
• Replicon: chromosome
• Accession: NC_017554
• Organism: Pantoea ananatis PA13

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: PAGR_RS08800
• Protein ID: WP_014605555.1
• Coordinates: 1884812..1885144 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: PAGR_RS08795
• Protein ID: WP_014605554.1
• Coordinates: 1884554..1884811 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PAGR_RS23050	1879837..1880262	+	426	WP_014605549.1	pentapeptide repeat-containing protein	-
PAGR_RS08775	1880550..1881788	-	1239	WP_014605550.1	MFS transporter	-
PAGR_RS08785	1882537..1883499	+	963	WP_014605551.1	ferrochelatase	-
PAGR_RS23060	1884001..1884206	+	206	Protein_1702	helix-turn-helix domain-containing protein	-
PAGR_RS08795	1884554..1884811	+	258	WP_014605554.1	antitoxin	Antitoxin
PAGR_RS08800	1884812..1885144	+	333	WP_014605555.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
PAGR_RS08810	1885405..1886238	-	834	WP_013026205.1	DgsA anti-repressor MtfA	-
PAGR_RS08815	1886344..1887021	-	678	WP_013026204.1	hypothetical protein	-
PAGR_RS08820	1887633..1888337	+	705	WP_014605556.1	phosphohydrolase	-
PAGR_RS08825	1888411..1888665	+	255	WP_013026202.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11931.63 Da
• Isoelectric Point: 6.9802

>T26993 WP_014605555.1 NC_017554:1884812-1885144 [Pantoea ananatis PA13]
MDRGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTL
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T26993 NC_017554:1884812-1885144 [Pantoea ananatis PA13]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGGATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCCTT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGAATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9253.73 Da
• Isoelectric Point: 4.5821

>AT26993 WP_014605554.1 NC_017554:1884554-1884811 [Pantoea ananatis PA13]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT26993 NC_017554:1884554-1884811 [Pantoea ananatis PA13]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCCCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGCTCCTGTAG