Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3834131..3834707 Replicon chromosome
Accession NZ_CP117020
Organism Escherichia coli strain MLI106K3

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NL424_RS18715 Protein ID WP_001297643.1
Coordinates 3834131..3834418 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag NL424_RS18720 Protein ID WP_000063160.1
Coordinates 3834405..3834707 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL424_RS18700 (3830368) 3830368..3831648 - 1281 WP_001037380.1 DUF445 domain-containing protein -
NL424_RS18705 (3831689) 3831689..3832921 - 1233 WP_001825915.1 multidrug efflux MFS transporter MdtM -
NL424_RS18710 (3833236) 3833236..3833932 + 697 Protein_3578 IS1 family transposase -
NL424_RS18715 (3834131) 3834131..3834418 + 288 WP_001297643.1 BrnT family toxin Toxin
NL424_RS18720 (3834405) 3834405..3834707 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
NL424_RS18725 (3834741) 3834741..3835697 - 957 WP_001297640.1 GTPase -
NL424_RS18730 (3835708) 3835708..3835911 - 204 WP_000467859.1 YbdD/YjiX family protein -
NL424_RS18735 (3835961) 3835961..3838111 - 2151 WP_001340937.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3833723..3833932 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T269868 WP_001297643.1 NZ_CP117020:3834131-3834418 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT269868 WP_000063160.1 NZ_CP117020:3834405-3834707 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References