Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3570329..3570826 Replicon chromosome
Accession NZ_CP117020
Organism Escherichia coli strain MLI106K3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL424_RS17450 Protein ID WP_001173343.1
Coordinates 3570653..3570826 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL424_RS17445 Protein ID WP_000398619.1
Coordinates 3570329..3570625 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL424_RS17425 (3567059) 3567059..3567778 - 720 WP_000611288.1 two-component system response regulator DcuR -
NL424_RS17430 (3567775) 3567775..3569406 - 1632 WP_001216477.1 sensor histidine kinase -
NL424_RS17435 (3569586) 3569586..3569816 + 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NL424_RS17440 (3569828) 3569828..3570100 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL424_RS17445 (3570329) 3570329..3570625 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL424_RS17450 (3570653) 3570653..3570826 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL424_RS17455 (3570945) 3570945..3572462 - 1518 WP_001550472.1 lysine--tRNA ligase -
NL424_RS17460 (3572699) 3572699..3574156 - 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269866 WP_001173343.1 NZ_CP117020:3570653-3570826 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269866 WP_000398619.1 NZ_CP117020:3570329-3570625 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References