Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2145908..2146562 Replicon chromosome
Accession NZ_CP117020
Organism Escherichia coli strain MLI106K3

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NL424_RS10710 Protein ID WP_000244781.1
Coordinates 2145908..2146315 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL424_RS10715 Protein ID WP_000354046.1
Coordinates 2146296..2146562 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL424_RS10690 (2141865) 2141865..2143598 - 1734 WP_001598594.1 single-stranded-DNA-specific exonuclease RecJ -
NL424_RS10695 (2143604) 2143604..2144314 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL424_RS10700 (2144339) 2144339..2145235 - 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
NL424_RS10705 (2145347) 2145347..2145868 + 522 WP_001055874.1 flavodoxin FldB -
NL424_RS10710 (2145908) 2145908..2146315 - 408 WP_000244781.1 protein YgfX Toxin
NL424_RS10715 (2146296) 2146296..2146562 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL424_RS10720 (2146805) 2146805..2147785 + 981 WP_001562036.1 tRNA-modifying protein YgfZ -
NL424_RS10725 (2147862) 2147862..2148521 - 660 WP_000250269.1 hemolysin III family protein -
NL424_RS10730 (2148685) 2148685..2148996 - 312 WP_001598596.1 N(4)-acetylcytidine aminohydrolase -
NL424_RS10735 (2149041) 2149041..2150474 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T269863 WP_000244781.1 NZ_CP117020:c2146315-2145908 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269863 WP_000354046.1 NZ_CP117020:c2146562-2146296 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References