Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3408438..3408935 Replicon chromosome
Accession NZ_CP117016
Organism Escherichia coli strain MLI121

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL415_RS17215 Protein ID WP_001173343.1
Coordinates 3408762..3408935 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag NL415_RS17210 Protein ID WP_001560287.1
Coordinates 3408438..3408734 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL415_RS17190 (3405167) 3405167..3405886 - 720 WP_000611288.1 two-component system response regulator DcuR -
NL415_RS17195 (3405883) 3405883..3407514 - 1632 WP_001216477.1 sensor histidine kinase -
NL415_RS17200 (3407695) 3407695..3407925 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
NL415_RS17205 (3407937) 3407937..3408209 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL415_RS17210 (3408438) 3408438..3408734 + 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL415_RS17215 (3408762) 3408762..3408935 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL415_RS17220 (3409054) 3409054..3410571 - 1518 WP_000003806.1 lysine--tRNA ligase -
NL415_RS17225 (3410808) 3410808..3412265 - 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269838 WP_001173343.1 NZ_CP117016:3408762-3408935 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT269838 WP_001560287.1 NZ_CP117016:3408438-3408734 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References