Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2776916..2777528 Replicon chromosome
Accession NZ_CP117016
Organism Escherichia coli strain MLI121

Toxin (Protein)


Gene name hicA Uniprot ID A0A167AMN3
Locus tag NL415_RS14355 Protein ID WP_000833474.1
Coordinates 2777343..2777528 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag NL415_RS14350 Protein ID WP_000499746.1
Coordinates 2776916..2777326 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL415_RS14335 (2772428) 2772428..2773243 - 816 WP_000891838.1 AraC family transcriptional regulator -
NL415_RS14340 (2773469) 2773469..2774869 + 1401 WP_000204815.1 MFS transporter -
NL415_RS14345 (2774880) 2774880..2776844 + 1965 WP_001026871.1 glycoside hydrolase family 127 protein -
NL415_RS14350 (2776916) 2776916..2777326 - 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
NL415_RS14355 (2777343) 2777343..2777528 - 186 WP_000833474.1 type II toxin-antitoxin system HicA family toxin Toxin
NL415_RS14360 (2778001) 2778001..2779080 + 1080 WP_000061479.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
NL415_RS14365 (2779121) 2779121..2780659 - 1539 WP_001469350.1 aldehyde dehydrogenase AldB -
NL415_RS14370 (2780860) 2780860..2782011 - 1152 WP_000741506.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6819.94 Da        Isoelectric Point: 12.0345

>T269837 WP_000833474.1 NZ_CP117016:c2777528-2777343 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSRHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT269837 WP_000499746.1 NZ_CP117016:c2777326-2776916 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167AMN3

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References