Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1870889..1871543 Replicon chromosome
Accession NZ_CP117016
Organism Escherichia coli strain MLI121

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NL415_RS09905 Protein ID WP_000244781.1
Coordinates 1870889..1871296 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL415_RS09910 Protein ID WP_000354046.1
Coordinates 1871277..1871543 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL415_RS09885 (1866846) 1866846..1868579 - 1734 WP_000813176.1 single-stranded-DNA-specific exonuclease RecJ -
NL415_RS09890 (1868585) 1868585..1869295 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL415_RS09895 (1869320) 1869320..1870216 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NL415_RS09900 (1870328) 1870328..1870849 + 522 WP_001055872.1 flavodoxin FldB -
NL415_RS09905 (1870889) 1870889..1871296 - 408 WP_000244781.1 protein YgfX Toxin
NL415_RS09910 (1871277) 1871277..1871543 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL415_RS09915 (1871786) 1871786..1872766 + 981 WP_000886075.1 tRNA-modifying protein YgfZ -
NL415_RS09920 (1872843) 1872843..1873502 - 660 WP_000250269.1 hemolysin III family protein -
NL415_RS09925 (1873666) 1873666..1873977 - 312 WP_001182958.1 N(4)-acetylcytidine aminohydrolase -
NL415_RS09930 (1874022) 1874022..1875455 + 1434 WP_034173731.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T269835 WP_000244781.1 NZ_CP117016:c1871296-1870889 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269835 WP_000354046.1 NZ_CP117016:c1871543-1871277 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References