Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 289234..290822 Replicon chromosome
Accession NZ_CP117016
Organism Escherichia coli strain MLI121

Toxin (Protein)


Gene name hipA Uniprot ID A0A377LF41
Locus tag NL415_RS02005 Protein ID WP_001125419.1
Coordinates 289234..290556 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A366Y5V8
Locus tag NL415_RS02010 Protein ID WP_001366357.1
Coordinates 290556..290822 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL415_RS01965 (284515) 284515..285087 - 573 WP_000789913.1 NAD(P)H-dependent oxidoreductase -
NL415_RS01970 (285238) 285238..285390 - 153 WP_001322417.1 amidohydrolase family protein -
NL415_RS01975 (285380) 285380..285799 - 420 WP_001327999.1 amidohydrolase family protein -
NL415_RS01980 (285759) 285759..285914 - 156 WP_001327998.1 hypothetical protein -
NL415_RS01985 (286017) 286017..286964 - 948 WP_000854795.1 aldo/keto reductase -
NL415_RS01990 (287038) 287038..287727 - 690 WP_000801313.1 type 1 glutamine amidotransferase domain-containing protein -
NL415_RS01995 (287736) 287736..288044 - 309 WP_001221046.1 hypothetical protein -
NL415_RS02000 (288184) 288184..289083 + 900 WP_001057775.1 LysR substrate-binding domain-containing protein -
NL415_RS02005 (289234) 289234..290556 - 1323 WP_001125419.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
NL415_RS02010 (290556) 290556..290822 - 267 WP_001366357.1 type II toxin-antitoxin system antitoxin HipB Antitoxin

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49317.90 Da        Isoelectric Point: 8.8783

>T269828 WP_001125419.1 NZ_CP117016:c290556-289234 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPNAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10077.48 Da        Isoelectric Point: 4.9632

>AT269828 WP_001366357.1 NZ_CP117016:c290822-290556 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLMTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377LF41

Antitoxin

Source ID Structure
AlphaFold DB A0A366Y5V8

References