Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2662882..2664470 Replicon chromosome
Accession NZ_CP117013
Organism Escherichia coli strain MLI114

Toxin (Protein)


Gene name hipA Uniprot ID A0A377LF41
Locus tag NL414_RS13610 Protein ID WP_001125419.1
Coordinates 2663148..2664470 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A366Y5V8
Locus tag NL414_RS13605 Protein ID WP_001366357.1
Coordinates 2662882..2663148 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL414_RS13605 (2662882) 2662882..2663148 + 267 WP_001366357.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
NL414_RS13610 (2663148) 2663148..2664470 + 1323 WP_001125419.1 type II toxin-antitoxin system serine/threonine protein kinase toxin HipA Toxin
NL414_RS13615 (2664621) 2664621..2665520 - 900 WP_001057775.1 LysR substrate-binding domain-containing protein -
NL414_RS13620 (2665660) 2665660..2665968 + 309 WP_001221046.1 hypothetical protein -
NL414_RS13625 (2665977) 2665977..2666666 + 690 WP_000801313.1 type 1 glutamine amidotransferase domain-containing protein -
NL414_RS13630 (2666740) 2666740..2667687 + 948 WP_000854795.1 aldo/keto reductase -
NL414_RS13635 (2667790) 2667790..2667945 + 156 WP_001327998.1 hypothetical protein -
NL414_RS13640 (2667905) 2667905..2668324 + 420 WP_001327999.1 amidohydrolase family protein -
NL414_RS13645 (2668314) 2668314..2668466 + 153 WP_001322417.1 amidohydrolase family protein -
NL414_RS13650 (2668617) 2668617..2669189 + 573 WP_000789913.1 NAD(P)H-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(7-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-69)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49317.90 Da        Isoelectric Point: 8.8783

>T269817 WP_001125419.1 NZ_CP117013:2663148-2664470 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLLPENETITRPIMAWEQLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPNAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Download         Length: 1323 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10077.48 Da        Isoelectric Point: 4.9632

>AT269817 WP_001366357.1 NZ_CP117013:2662882-2663148 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLMTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377LF41

Antitoxin

Source ID Structure
AlphaFold DB A0A366Y5V8

References