Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1190695..1191278 Replicon chromosome
Accession NZ_CP117013
Organism Escherichia coli strain MLI114

Toxin (Protein)


Gene name mazF Uniprot ID A0A377LAP4
Locus tag NL414_RS06145 Protein ID WP_000254736.1
Coordinates 1190943..1191278 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NL414_RS06140 Protein ID WP_000581937.1
Coordinates 1190695..1190943 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL414_RS06130 (1187034) 1187034..1188335 + 1302 WP_000046786.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
NL414_RS06135 (1188383) 1188383..1190617 + 2235 WP_000226799.1 GTP diphosphokinase -
NL414_RS06140 (1190695) 1190695..1190943 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NL414_RS06145 (1190943) 1190943..1191278 + 336 WP_000254736.1 endoribonuclease MazF Toxin
NL414_RS06150 (1191349) 1191349..1192140 + 792 WP_001071667.1 nucleoside triphosphate pyrophosphohydrolase -
NL414_RS06155 (1192367) 1192367..1194004 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NL414_RS06160 (1194092) 1194092..1195390 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12125.11 Da        Isoelectric Point: 8.7181

>T269811 WP_000254736.1 NZ_CP117013:1190943-1191278 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGKVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT269811 WP_000581937.1 NZ_CP117013:1190695-1190943 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377LAP4

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References