Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1070961..1071615 Replicon chromosome
Accession NZ_CP117013
Organism Escherichia coli strain MLI114

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NL414_RS05645 Protein ID WP_000244781.1
Coordinates 1071208..1071615 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL414_RS05640 Protein ID WP_000354046.1
Coordinates 1070961..1071227 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL414_RS05620 (1067049) 1067049..1068482 - 1434 WP_034173731.1 6-phospho-beta-glucosidase BglA -
NL414_RS05625 (1068527) 1068527..1068838 + 312 WP_001182958.1 N(4)-acetylcytidine aminohydrolase -
NL414_RS05630 (1069002) 1069002..1069661 + 660 WP_000250269.1 hemolysin III family protein -
NL414_RS05635 (1069738) 1069738..1070718 - 981 WP_000886075.1 tRNA-modifying protein YgfZ -
NL414_RS05640 (1070961) 1070961..1071227 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL414_RS05645 (1071208) 1071208..1071615 + 408 WP_000244781.1 protein YgfX Toxin
NL414_RS05650 (1071655) 1071655..1072176 - 522 WP_001055872.1 flavodoxin FldB -
NL414_RS05655 (1072288) 1072288..1073184 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NL414_RS05660 (1073209) 1073209..1073919 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL414_RS05665 (1073925) 1073925..1075658 + 1734 WP_000813176.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T269810 WP_000244781.1 NZ_CP117013:1071208-1071615 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269810 WP_000354046.1 NZ_CP117013:1070961-1071227 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References