Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4701483..4701980 Replicon chromosome
Accession NZ_CP117008
Organism Escherichia coli strain MLI109

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL412_RS23820 Protein ID WP_001173343.1
Coordinates 4701483..4701656 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL412_RS23825 Protein ID WP_000398619.1
Coordinates 4701684..4701980 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL412_RS23810 (4698153) 4698153..4699610 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NL412_RS23815 (4699847) 4699847..4701364 + 1518 WP_001550472.1 lysine--tRNA ligase -
NL412_RS23820 (4701483) 4701483..4701656 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL412_RS23825 (4701684) 4701684..4701980 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL412_RS23830 (4702209) 4702209..4702481 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL412_RS23835 (4702493) 4702493..4702723 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NL412_RS23840 (4702903) 4702903..4704534 + 1632 WP_001216477.1 sensor histidine kinase -
NL412_RS23845 (4704531) 4704531..4705250 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269785 WP_001173343.1 NZ_CP117008:c4701656-4701483 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269785 WP_000398619.1 NZ_CP117008:c4701980-4701684 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References