Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3203805..3204589 Replicon chromosome
Accession NZ_CP117008
Organism Escherichia coli strain MLI109

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag NL412_RS16785 Protein ID WP_000613626.1
Coordinates 3204095..3204589 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag NL412_RS16780 Protein ID WP_001110447.1
Coordinates 3203805..3204098 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL412_RS16770 (3198955) 3198955..3199914 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
NL412_RS16775 (3200487) 3200487..3203672 + 3186 WP_001550951.1 ribonuclease E -
NL412_RS16780 (3203805) 3203805..3204098 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
NL412_RS16785 (3204095) 3204095..3204589 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
NL412_RS16790 (3204684) 3204684..3205637 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
NL412_RS16795 (3205649) 3205649..3207292 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
NL412_RS16800 (3207358) 3207358..3208299 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
NL412_RS16805 (3208299) 3208299..3209396 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T269778 WP_000613626.1 NZ_CP117008:3204095-3204589 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT269778 WP_001110447.1 NZ_CP117008:3203805-3204098 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References