Detailed information of TA system TA026976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 123835..124586
• Replicon: plasmid pKCTC2242
• Accession: NC_017541
• Organism: Klebsiella pneumoniae KCTC 2242

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: KPN2242_RS26740
• Protein ID: WP_004902249.1
• Coordinates: 123835..124317 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: KPN2242_RS26745
• Protein ID: WP_004902250.1
• Coordinates: 124308..124586 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPN2242_RS26715	118841..119809	+	969	WP_004225014.1	IS5 family transposase	-
KPN2242_RS28445	119866..120102	+	237	Protein_123	kinase	-
KPN2242_RS26720	120234..120881	-	648	WP_014386537.1	EcsC family protein	-
KPN2242_RS26725	120908..121663	-	756	WP_004902235.1	DUF2971 domain-containing protein	-
KPN2242_RS29350	121764..122156	-	393	WP_045145491.1	hypothetical protein	-
KPN2242_RS26730	122261..122800	-	540	WP_014599321.1	hypothetical protein	-
KPN2242_RS26740	123835..124317	-	483	WP_004902249.1	GNAT family N-acetyltransferase	Toxin
KPN2242_RS26745	124308..124586	-	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
KPN2242_RS26750	124705..124917	-	213	WP_004902255.1	hypothetical protein	-
KPN2242_RS26755	125025..125366	-	342	WP_004902257.1	hypothetical protein	-
KPN2242_RS28455	125826..126095	-	270	WP_071591895.1	hypothetical protein	-
KPN2242_RS26760	126333..128003	-	1671	WP_004902261.1	AMP-binding protein	-
KPN2242_RS26765	127984..129249	-	1266	WP_004210292.1	MSMEG_0569 family flavin-dependent oxidoreductase	-
KPN2242_RS26770	129267..129557	-	291	WP_011154627.1	MSMEG_0570 family nitrogen starvation response protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..202852	202852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17689.52 Da
• Isoelectric Point: 9.2033

>T26976 WP_004902249.1 NC_017541:c124317-123835 [Klebsiella pneumoniae KCTC 2242]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNKVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T26976 NC_017541:c124317-123835 [Klebsiella pneumoniae KCTC 2242]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACAAAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT26976 WP_004902250.1 NC_017541:c124586-124308 [Klebsiella pneumoniae KCTC 2242]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT26976 NC_017541:c124586-124308 [Klebsiella pneumoniae KCTC 2242]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A071LPN3