Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4395049..4395546 Replicon chromosome
Accession NZ_CP117006
Organism Escherichia coli strain MLI108K3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL411_RS22965 Protein ID WP_001173343.1
Coordinates 4395049..4395222 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL411_RS22970 Protein ID WP_000398619.1
Coordinates 4395250..4395546 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL411_RS22955 (4391719) 4391719..4393176 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
NL411_RS22960 (4393413) 4393413..4394930 + 1518 WP_001550472.1 lysine--tRNA ligase -
NL411_RS22965 (4395049) 4395049..4395222 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL411_RS22970 (4395250) 4395250..4395546 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL411_RS22975 (4395775) 4395775..4396047 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NL411_RS22980 (4396059) 4396059..4396289 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
NL411_RS22985 (4396469) 4396469..4398100 + 1632 WP_001216477.1 sensor histidine kinase -
NL411_RS22990 (4398097) 4398097..4398816 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269755 WP_001173343.1 NZ_CP117006:c4395222-4395049 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269755 WP_000398619.1 NZ_CP117006:c4395546-4395250 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References