Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4211786..4212362 Replicon chromosome
Accession NZ_CP117006
Organism Escherichia coli strain MLI108K3

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NL411_RS22055 Protein ID WP_001297643.1
Coordinates 4212075..4212362 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag NL411_RS22050 Protein ID WP_000063160.1
Coordinates 4211786..4212088 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL411_RS22035 (4208382) 4208382..4210532 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
NL411_RS22040 (4210582) 4210582..4210785 + 204 WP_000467859.1 YbdD/YjiX family protein -
NL411_RS22045 (4210796) 4210796..4211752 + 957 WP_001297640.1 GTPase -
NL411_RS22050 (4211786) 4211786..4212088 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
NL411_RS22055 (4212075) 4212075..4212362 - 288 WP_001297643.1 BrnT family toxin Toxin
NL411_RS22060 (4212561) 4212561..4213257 - 697 Protein_3996 IS1 family transposase -
NL411_RS22065 (4213572) 4213572..4214804 + 1233 WP_001825915.1 multidrug efflux MFS transporter MdtM -
NL411_RS22070 (4214845) 4214845..4216125 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4212561..4212770 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T269754 WP_001297643.1 NZ_CP117006:c4212362-4212075 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT269754 WP_000063160.1 NZ_CP117006:c4212088-4211786 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References