Detailed information of TA system TA026974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 14185..14855
• Replicon: plasmid pKCTC2242
• Accession: NC_017541
• Organism: Klebsiella pneumoniae KCTC 2242

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: KPN2242_RS26200
• Protein ID: WP_004213072.1
• Coordinates: 14412..14855 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: KPN2242_RS26195
• Protein ID: WP_004213073.1
• Coordinates: 14185..14415 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPN2242_RS26170	10408..11307	-	900	WP_004225022.1	nucleotide-binding protein	-
KPN2242_RS26175	11297..11587	-	291	WP_004213078.1	hypothetical protein	-
KPN2242_RS26180	11939..12145	+	207	WP_004213077.1	hypothetical protein	-
KPN2242_RS26185	12135..12428	-	294	WP_004213076.1	hypothetical protein	-
KPN2242_RS26190	12444..13577	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
KPN2242_RS26195	14185..14415	+	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KPN2242_RS26200	14412..14855	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
KPN2242_RS26205	14869..15285	-	417	Protein_16	restriction endonuclease	-
KPN2242_RS26210	15289..15582	-	294	Protein_17	transposase	-
KPN2242_RS29875	15999..16635	+	637	Protein_18	mucoid phenotype A regulator RmpA	-
KPN2242_RS28365	17150..17556	-	407	Protein_19	GAF domain-containing protein	-
KPN2242_RS26220	17647..18567	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
KPN2242_RS26225	18616..19107	-	492	WP_011251327.1	DM13 domain-containing protein	-
KPN2242_RS26230	19170..19445	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..202852	202852

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T26974 WP_004213072.1 NC_017541:14412-14855 [Klebsiella pneumoniae KCTC 2242]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T26974 NC_017541:14412-14855 [Klebsiella pneumoniae KCTC 2242]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT26974 WP_004213073.1 NC_017541:14185-14415 [Klebsiella pneumoniae KCTC 2242]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT26974 NC_017541:14185-14415 [Klebsiella pneumoniae KCTC 2242]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620