Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5040074..5040699 Replicon chromosome
Accession NC_017540
Organism Klebsiella pneumoniae KCTC 2242

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KPN2242_RS25020 Protein ID WP_002882817.1
Coordinates 5040316..5040699 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KPN2242_RS25015 Protein ID WP_004150355.1
Coordinates 5040074..5040316 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPN2242_RS24990 5036065..5036664 + 600 WP_004901669.1 glucose-1-phosphatase -
KPN2242_RS24995 5036658..5037518 + 861 WP_002882807.1 virulence factor BrkB family protein -
KPN2242_RS25000 5037515..5037952 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
KPN2242_RS25005 5037997..5038938 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KPN2242_RS25010 5038952..5039869 - 918 WP_004901674.1 alpha/beta hydrolase -
KPN2242_RS25015 5040074..5040316 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
KPN2242_RS25020 5040316..5040699 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KPN2242_RS25025 5040873..5041802 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KPN2242_RS25030 5041799..5042434 - 636 WP_004901683.1 formate dehydrogenase cytochrome b556 subunit -
KPN2242_RS25035 5042431..5043333 - 903 WP_004901686.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T26973 WP_002882817.1 NC_017540:5040316-5040699 [Klebsiella pneumoniae KCTC 2242]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T26973 NC_017540:5040316-5040699 [Klebsiella pneumoniae KCTC 2242]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT26973 WP_004150355.1 NC_017540:5040074-5040316 [Klebsiella pneumoniae KCTC 2242]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT26973 NC_017540:5040074-5040316 [Klebsiella pneumoniae KCTC 2242]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References