Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 33574..34372 Replicon plasmid p108_MLI108-3
Accession NZ_CP117002
Organism Escherichia coli strain MLI108K3

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag NL411_RS00205 Protein ID WP_000072677.1
Coordinates 33851..34372 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag NL411_RS00200 Protein ID WP_042063163.1
Coordinates 33574..33843 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL411_RS00175 (NL411_000175) 28998..30338 - 1341 WP_094308010.1 DnaB-like helicase C-terminal domain-containing protein -
NL411_RS00180 (NL411_000180) 30382..31122 - 741 WP_094308011.1 hypothetical protein -
NL411_RS00185 (NL411_000185) 31405..32172 + 768 WP_000342417.1 hypothetical protein -
NL411_RS00190 (NL411_000190) 32228..32581 - 354 WP_160378290.1 hypothetical protein -
NL411_RS00195 (NL411_000195) 32587..33255 - 669 WP_042063113.1 division plane positioning ATPase MipZ -
NL411_RS00200 (NL411_000200) 33574..33843 + 270 WP_042063163.1 DUF1778 domain-containing protein Antitoxin
NL411_RS00205 (NL411_000205) 33851..34372 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
NL411_RS00210 (NL411_000210) 34541..34792 - 252 WP_001404395.1 hypothetical protein -
NL411_RS00215 (NL411_000215) 34794..35486 - 693 WP_042063115.1 membrane protein -
NL411_RS00220 (NL411_000220) 35500..35823 - 324 WP_000064175.1 hypothetical protein -
NL411_RS00225 (NL411_000225) 36005..36289 - 285 WP_021520115.1 hypothetical protein -
NL411_RS00230 (NL411_000230) 36300..37004 - 705 WP_000235971.1 tail fiber domain-containing protein -
NL411_RS00235 (NL411_000235) 37014..37295 - 282 WP_042063118.1 hypothetical protein -
NL411_RS00240 (NL411_000240) 37295..39205 - 1911 Protein_44 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fdeC 1..108353 108353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T269728 WP_000072677.1 NZ_CP117002:33851-34372 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10081.55 Da        Isoelectric Point: 8.7353

>AT269728 WP_042063163.1 NZ_CP117002:33574-33843 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure

References