Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3757908..3758484 Replicon chromosome
Accession NZ_CP117001
Organism Escherichia coli strain MLI108K2

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NL410_RS20240 Protein ID WP_001297643.1
Coordinates 3757908..3758195 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NL410_RS20245 Protein ID WP_000063156.1
Coordinates 3758182..3758484 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL410_RS20220 (3754161) 3754161..3755441 - 1281 WP_001037380.1 DUF445 domain-containing protein -
NL410_RS20225 (3755482) 3755482..3756714 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
NL410_RS20230 (3756860) 3756860..3756955 - 96 Protein_3603 dienelactone hydrolase family protein -
NL410_RS20235 (3757012) 3757012..3757709 + 698 Protein_3604 IS1 family transposase -
NL410_RS20240 (3757908) 3757908..3758195 + 288 WP_001297643.1 BrnT family toxin Toxin
NL410_RS20245 (3758182) 3758182..3758484 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NL410_RS20250 (3758518) 3758518..3759474 - 957 WP_001297640.1 GTPase -
NL410_RS20255 (3759485) 3759485..3759688 - 204 WP_000467859.1 YbdD/YjiX family protein -
NL410_RS20260 (3759738) 3759738..3761888 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T269724 WP_001297643.1 NZ_CP117001:3757908-3758195 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT269724 WP_000063156.1 NZ_CP117001:3758182-3758484 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References