Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4760681..4761420 Replicon chromosome
Accession NC_017540
Organism Klebsiella pneumoniae KCTC 2242

Toxin (Protein)


Gene name tacT Uniprot ID A0A4U9WP91
Locus tag KPN2242_RS23645 Protein ID WP_004901283.1
Coordinates 4760681..4761166 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KPN2242_RS23650 Protein ID WP_003026799.1
Coordinates 4761154..4761420 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPN2242_RS23620 4756309..4756599 + 291 WP_002921931.1 HTH-type transcriptional regulator -
KPN2242_RS23625 4756851..4757063 + 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
KPN2242_RS23630 4757162..4758781 - 1620 WP_004901278.1 ATP-binding cassette domain-containing protein -
KPN2242_RS23635 4759083..4759235 - 153 WP_002922102.1 type I toxin-antitoxin system toxin HokA -
KPN2242_RS23640 4759309..4760356 + 1048 Protein_4525 IS481-like element ISKpn28 family transposase -
KPN2242_RS23645 4760681..4761166 - 486 WP_004901283.1 GNAT family N-acetyltransferase Toxin
KPN2242_RS23650 4761154..4761420 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KPN2242_RS28215 4761552..4761980 - 429 WP_004901287.1 GFA family protein -
KPN2242_RS23665 4763846..4765915 - 2070 WP_004901297.1 glycine--tRNA ligase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 4759309..4762906 3597


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17594.32 Da        Isoelectric Point: 10.1788

>T26972 WP_004901283.1 NC_017540:c4761166-4760681 [Klebsiella pneumoniae KCTC 2242]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLNQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Download         Length: 486 bp

>T26972 NC_017540:c4761166-4760681 [Klebsiella pneumoniae KCTC 2242]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT26972 WP_003026799.1 NC_017540:c4761420-4761154 [Klebsiella pneumoniae KCTC 2242]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT26972 NC_017540:c4761420-4761154 [Klebsiella pneumoniae KCTC 2242]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4U9WP91

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References