Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2159915..2160569 Replicon chromosome
Accession NZ_CP117001
Organism Escherichia coli strain MLI108K2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NL410_RS12445 Protein ID WP_000244772.1
Coordinates 2159915..2160322 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL410_RS12450 Protein ID WP_000354046.1
Coordinates 2160303..2160569 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL410_RS12425 (2155872) 2155872..2157605 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
NL410_RS12430 (2157611) 2157611..2158321 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL410_RS12435 (2158346) 2158346..2159242 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NL410_RS12440 (2159354) 2159354..2159875 + 522 WP_001055874.1 flavodoxin FldB -
NL410_RS12445 (2159915) 2159915..2160322 - 408 WP_000244772.1 protein YgfX Toxin
NL410_RS12450 (2160303) 2160303..2160569 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL410_RS12455 (2160812) 2160812..2161792 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NL410_RS12460 (2161869) 2161869..2162528 - 660 WP_000250274.1 hemolysin III family protein -
NL410_RS12465 (2162692) 2162692..2163003 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NL410_RS12470 (2163048) 2163048..2164481 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
NL410_RS12475 (2164538) 2164538..2165281 - 744 WP_000951940.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T269719 WP_000244772.1 NZ_CP117001:c2160322-2159915 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269719 WP_000354046.1 NZ_CP117001:c2160569-2160303 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References