Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3757272..3757848 Replicon chromosome
Accession NZ_CP116994
Organism Escherichia coli strain MLI108K1

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag NL409_RS20225 Protein ID WP_001297643.1
Coordinates 3757272..3757559 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag NL409_RS20230 Protein ID WP_000063156.1
Coordinates 3757546..3757848 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL409_RS20205 (3753525) 3753525..3754805 - 1281 WP_001037380.1 DUF445 domain-containing protein -
NL409_RS20210 (3754846) 3754846..3756078 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
NL409_RS20215 (3756224) 3756224..3756319 - 96 Protein_3600 dienelactone hydrolase family protein -
NL409_RS20220 (3756376) 3756376..3757073 + 698 Protein_3601 IS1 family transposase -
NL409_RS20225 (3757272) 3757272..3757559 + 288 WP_001297643.1 BrnT family toxin Toxin
NL409_RS20230 (3757546) 3757546..3757848 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
NL409_RS20235 (3757882) 3757882..3758838 - 957 WP_001297640.1 GTPase -
NL409_RS20240 (3758849) 3758849..3759052 - 204 WP_000467859.1 YbdD/YjiX family protein -
NL409_RS20245 (3759102) 3759102..3761252 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T269693 WP_001297643.1 NZ_CP116994:3757272-3757559 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT269693 WP_000063156.1 NZ_CP116994:3757546-3757848 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References