Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3493621..3494118 Replicon chromosome
Accession NZ_CP116994
Organism Escherichia coli strain MLI108K1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NL409_RS18865 Protein ID WP_001173343.1
Coordinates 3493945..3494118 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NL409_RS18860 Protein ID WP_000398619.1
Coordinates 3493621..3493917 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL409_RS18840 (3490352) 3490352..3491071 - 720 WP_000611288.1 two-component system response regulator DcuR -
NL409_RS18845 (3491068) 3491068..3492699 - 1632 WP_001216477.1 sensor histidine kinase -
NL409_RS18850 (3492880) 3492880..3493110 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NL409_RS18855 (3493122) 3493122..3493394 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
NL409_RS18860 (3493621) 3493621..3493917 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NL409_RS18865 (3493945) 3493945..3494118 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NL409_RS18870 (3494237) 3494237..3495754 - 1518 WP_001295074.1 lysine--tRNA ligase -
NL409_RS18875 (3495991) 3495991..3497448 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T269690 WP_001173343.1 NZ_CP116994:3493945-3494118 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT269690 WP_000398619.1 NZ_CP116994:3493621-3493917 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References