Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2159159..2159813 Replicon chromosome
Accession NZ_CP116994
Organism Escherichia coli strain MLI108K1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NL409_RS12430 Protein ID WP_000244772.1
Coordinates 2159159..2159566 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NL409_RS12435 Protein ID WP_000354046.1
Coordinates 2159547..2159813 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NL409_RS12410 (2155116) 2155116..2156849 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
NL409_RS12415 (2156855) 2156855..2157565 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NL409_RS12420 (2157590) 2157590..2158486 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NL409_RS12425 (2158598) 2158598..2159119 + 522 WP_001055874.1 flavodoxin FldB -
NL409_RS12430 (2159159) 2159159..2159566 - 408 WP_000244772.1 protein YgfX Toxin
NL409_RS12435 (2159547) 2159547..2159813 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NL409_RS12440 (2160056) 2160056..2161036 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NL409_RS12445 (2161113) 2161113..2161772 - 660 WP_000250274.1 hemolysin III family protein -
NL409_RS12450 (2161936) 2161936..2162247 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NL409_RS12455 (2162292) 2162292..2163725 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
NL409_RS12460 (2163782) 2163782..2164525 - 744 WP_000951940.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T269688 WP_000244772.1 NZ_CP116994:c2159566-2159159 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT269688 WP_000354046.1 NZ_CP116994:c2159813-2159547 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References